The Identification and Differentiation between Burkholderia mallei and Burkholderia pseudomallei Using One Gene Pyrosequencing

Table 4

Results of PCR and pyrosequencing assays for B. mallei and B. pseudomallei.

Organism name (number)	B. mallei Assays		B. pseudomallei Assays
	PCR	Pyrosequence	PCR	Pyrosequence
B. mallei (10)	+	PASS	−	FAIL
B. pseudomallei (10)	−	FAIL	+	PASS
B. cepacia (15)	−	FAIL	−	FAIL
Burkholderia species (8)	−	FAIL	−	FAIL
Other Bacteria (17)
Gram negative strains (10)	−	FAIL	−	FAIL
Gram positive strains (7)	−	FAIL	−	FAIL

“+” denotes a positive result or amplicon was produced.
“−” denotes a negative results.
“PASS” denotes the pyrosequencing assay produced a reliable sequence.
“Fail” denotes the pyrosequencing assay produced no reliable sequence or no sequence at all.