Table of Contents
International Scholarly Research Notices
Volume 2014, Article ID 128120, 3 pages
Research Article

False-Positive Clostridium difficile in Negative-Control Reactions Peak and Then Decrease with Repetitive Refrigeration of Immunoassay

1Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY 14580, USA
2Division of Gastroenterology and Liver Disease, Case Western Reserve University, Cleveland, OH 44106, USA
3Department of Clinical Studies, University of Guelph, Guelph, ON, Canada N1G 2W1

Received 14 May 2014; Accepted 25 June 2014; Published 11 September 2014

Academic Editor: Isabel Gómez

Copyright © 2014 Alexander Rodriguez-Palacios et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aberrant false-positive reactions in negative-controls during ELISA testing for Clostridium difficile indicated the potential for false-diagnoses. Experiments with 96-well products showed a maximum peak of false-positive immunoassay reactions with the provided negative-control reagents after 5 refrigeration-to-room temperature cycles (), decreasing thereafter with additional refrigeration cycles. Because repetitive refrigeration causes a peak of false-positives, the use of single negative-controls per ELISA run might be insufficient to monitor aberrant preanalytical false-positives if immunoassays are subject to repetitive refrigeration.