Table of Contents
ISRN Neuroscience
Volume 2014, Article ID 163459, 8 pages
http://dx.doi.org/10.1155/2014/163459
Research Article

Prenatal Exposure to Lamotrigine: Effects on Postnatal Development and Behaviour in Rat Offspring

1Centre for Toxicology and Developmental Research (CEFT), Sri Ramachandra University, Chennai, Tamil Nadu 600116, India
2Department of Biochemistry, Sri Ramachandra University, Chennai, Tamil Nadu 600116, India
3School of Chemical and Biotechnology, Shanmugha Arts, Science, Technology and Research Academy (SASTRA University), Thanjavur, Tamil Nadu 613402, India

Received 11 January 2014; Accepted 19 February 2014; Published 14 April 2014

Academic Editors: I. Berger, A. M. Depino, and R. Kulesza

Copyright © 2014 Sekar Sathiya et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Use of antiepileptic drugs (AEDs) in pregnancy warrants various side effects and also deleterious effects on fetal development. The present study was carried out to assess the effects of prenatal exposure to lamotrigine (LTG) on postnatal development and behavioural alterations of offspring. Adult male and female Sprague Dawley rats weighing 150–180 g b. wt. were allowed to copulate and pregnancy was confirmed by vaginal cytology. Pregnant rats were treated with LTG (11.5, 23, and 46 mg/kg, p.o) from gestational day 3 (GND 3) and this treatment continued till postnatal day 11 (PND 11). Offspring were separated from their dam on day 21 following parturition. LTG, at 46 mg/kg, p.o, produced severe clinical signs of toxicity leading to death of dam between GND 15 and 17. LTG, at 11.5 and 23 mg/kg, p.o, showed significant alterations in offspring’s incisors eruption and vaginal opening when compared to age matched controls. LTG (23 mg/kg, p.o) exposed female offspring expressed hyperactive behaviour and decreased GABA-A receptor expression when compared to control rats. These results reveal that prenatal exposure to LTG may impart differential postnatal behavioural alterations between male and female rats which paves way for further investigations.