Table of Contents
ISRN Obstetrics and Gynecology
Volume 2014, Article ID 170124, 6 pages
Research Article

Environmental Tobacco Smoke Exposure during Intrauterine Period, Promotes Caspase Dependent and Independent DNA Fragmentation in Sertoli-Germ Cells

1Dumlupinar University, School of Medicine, Department of Obstetrics and Gynecology, 43000 Kutahya, Turkey
2Dr. Zekai Tahir BURAK Women Health, Education and Research Hospital, Department of Reproductive Endocrinology and IVF, Cebeci, 06230 Ankara, Turkey
3Gazi University, Faculty of Medicine, NanoMedicine Research Laboratory, Golbasi, 06810 Ankara, Turkey
4Namik Kemal University, Faculty of Medicine, 59000 Tekirdag, Turkey
5HRS Women Health and IVF Center, Cankaya, 06700 Ankara, Turkey
6Samsun 19 Mayıs University, Faculty of Medicine, 55220 Samsun, Turkey

Received 26 November 2013; Accepted 22 January 2014; Published 12 March 2014

Academic Editors: R. J. Chen and S. B. W. Yeung

Copyright © 2014 Beril Yüksel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objectives. To investigate the effect of cigarette smoke exposure during intrauterine period on neonatal rat testis. Methods. Twenty-five rats were randomized to be exposed to cigarette smoke with the Walton Smoking Machine or to room air during their pregnancies. The newborn male rats () were grouped as group 1 () which were exposed to cigarette smoke during intrauterine life and group 2 () which were exposed to room air during intrauterine life. The orchiectomy materials were analyzed with TUNEL immunofluorescent staining for detection of DNA damage. To detect apoptosis, immunohistochemical analyses with caspase-3 were performed. Primary outcomes were apoptotic index and immunohistochemical scores (HSCORES); secondary outcomes were Sertoli-cell count and birth-weight of rats. Results. Sertoli cell apoptosis was increased in group 1 (HSCORE ) when compared to group 2 (HSCORE ) (). Sertoli cell count was decreased in group 1 (). The HSCORE for the germ cells was calculated as in group 1 and in group 2 () referring to an increased germ cell apoptosis in group 1. The apoptotic indexes for group 1 were and for group 2 (). The immunofluorescent technique demonstrated increased DNA damage in seminiferous epithelium in group 1. Conclusions. Intrauterine exposure to cigarette smoke adversely affects neonatal testicular structuring and diminishes testicular reserve.