Table of Contents
International Scholarly Research Notices
Volume 2014 (2014), Article ID 185272, 6 pages
http://dx.doi.org/10.1155/2014/185272
Research Article

Molecular Typing of Hospital-Acquired Staphylococcus aureus Isolated from Isfahan, Iran

1Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2Department of Microbiology, Faculty of Bioscience, Islamic Azad University, Falavarjan Branch, Isfahan, Iran
3Department of Molecular and Cell Biochemistry, Faculty of Bioscience, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
4Department of Pathobiology and Anatomy, School of Medicine, North Khorasan University of Medical Science, Bojnord, Iran
5Department of Medical Bacteriology and Virology, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
6Department of Biology, Faculty of Sciences, Shiraz University, Shiraz, Iran
7Department of Microbiology, School of Medicine, Ahvaz University of Medical Sciences, Ahvaz, Iran

Received 2 July 2014; Accepted 12 October 2014; Published 9 November 2014

Academic Editor: Rupendra Jadhav

Copyright © 2014 Seyed Asghar Havaei et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Staphylococcus aureus (S. aureus) is one of the most common pathogens that cause hospital- and community-acquired infections in the world. The use of molecular typing methods is essential for determining the origin of the strains, their clonal relations, and also in epidemiological investigations. The purpose of this study was to determine the prevalence of antibiotic resistant S. aureus isolates and using spa, agr, and SCCmec typing to determine the dominant types in Iran. Material and Method. Fifty isolates of S. aureus were collected from January to May 2010. S. aureus identification was performed by biochemical tests. Disk diffusion method was employed to assess the sensitivity of S. aureus strains to antibiotics and then genetic analysis of bacteria was performed using SCCmec, agr, and spa typing. Results. S. aureus resistance to tetracycline, cefoxitin, clindamycin, ciprofloxacin, gentamicin, Cot: cotrimoxazole, levofloxacin, rifampin, and vancomycin were found to be 36%, 18%, 12%, 12%, 22%, 6%, 6%, and 0%, respectively. The results of this study showed that 16% of the isolates were resistant to methicillin (MRSA) and the majority of isolates were SSC mec type IV. In addition spa and agr typing revealed agr typeI and spa type t7688 to be the most predominant. Conclusion. In this study, spa typing showed 100% reliability and the t7688 spa type had a frequency of 26% compared to the frequency of 0.0% in the Ridom SpaServer. The frequency of t304 spa type was higher than the global average.