Table of Contents
ISRN Dentistry
Volume 2014, Article ID 191068, 4 pages
Research Article

In Vitro Cytotoxicity Evaluation of Four Vital Pulp Therapy Materials on L929 Fibroblasts

1Department of Bioengineering, University of Texas at Arlington, Arlington, TX 76019, USA
2Joint Biomedical Engineering Program, University of Texas at Arlington and University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
3Department of Clinical Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390-9066, USA
4Division of Endodontology, University of Connecticut Health Center, Farmington, CT 06030-1715, USA
5Department of Endodontics, West Virginia University School of Dentistry, One Medical Center Drive, P.O. Box 9450, Health Science Center North, Morgantown, WV 26506-9450, USA

Received 24 December 2013; Accepted 21 January 2014; Published 3 March 2014

Academic Editors: A. Merchant and P. Pereira

Copyright © 2014 Aniket S. Wadajkar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this study was to evaluate cytotoxicity of direct pulp capping materials such as Dycal, Life, ProRoot MTA, and Super-Bond C&B on L929 fibroblasts. Freshly mixed or set materials were prepared and eluted by incubation with cell culture medium for working time period (fresh) or for 6 hours (set). The cells were exposed to media containing elutes for 24 hours, after which the cell survival was evaluated by MTS assays. In freshly mixed materials, average ± standard deviation % cell viabilities were 40.2 ± 14.0%, 43.7 ± 16.0%, 72.9 ± 12.7%, and 66.0 ± 13.6% for Dycal, Life, ProRoot MTA, and Super-Bond C&B, respectively. There was no statistical difference in cell viabilities among material groups, whereas in set materials, the cell viabilities were 48.7 ± 14.8%, 37.2 ± 10.6%, 46.7 ± 15.2%, and 100 ± 21.9% for Dycal, Life, ProRoot MTA, and Super-Bond C&B, respectively. Super-Bond C&B showed more cell viabilities than the other three material groups . The four vital pulp therapy materials had similar cytotoxicity when the materials were fresh. Super-Bond C&B was less cytotoxic than Dycal, Life, and ProRoot MTA after the materials were set, which suggests the use of SB-C&B in future in vivo clinical investigations.