Identification of pBC218/pBC210 Genes of Bacillus cereus G9241 in Five Florida Soils Using qPCR

Table 4

Average CT^a value (and CT ranges) produced by DNA extracts of the five Florida soil samples positive for pBC210.

Sample number	CT value (range) for assay target ORF^b of pBC210			B. anthracis plasmids^c
	ORF 0047	ORF 0072	ORF 0067	pX01	pX02
7	37.21 (36.36–38.44)	36.39 (35.55–37.34)	36.43 (36.43–36.43)	−	−
8	37.67 (36.79–38.80)	37.15 (35.39–38.73)	36.00 (35.93–36.67)	−	−
14	38.44 (37.13–38.15)	36.52 (36.18–37.50)	36.25 (35.42–36.08)	−	−
39	35.91 (35.46–37.29)	35.16 (34.36–38.35)	34.99 (34.47–35.52)	+	−
42	38.84 (36.89–38.87)	36.51 (33.71–38.57)	28.81 (28.59–29.05)	+	−

a“CT” denotes cycle threshold value.
^bThe five soils obtained from Florida yielded positive results for ORF 0047 and ORF 0072. Subsequently, the soils were also tested with the primer and probe set for ORF 0067 for confirmation. All assays were performed in duplicate in multiple runs and by two different personnel in order to rule out errors.
^cSoils had been previously tested for the genes lef, pag, and cya of pX01 and capC on pX02 of B. anthracis as previously reported [16–18].