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This paper has been retracted as it was submitted for publication without the knowledge and approval of one of the co-authors, Dr Suman Lata Mendiratta. The submitting author Dr. Dipti Kalita plagiarized data from the Thalassemia control Project headed by Dr Suman Lata Mendiratta.

International Scholarly Research Notices
Volume 2014 (2014), Article ID 257805, 10 pages
Research Article

Detection of Abnormal Hemoglobin Variants by HPLC Method: Common Problems with Suggested Solutions

1Department of Pathology, Hindu Rao Hospital, Delhi 110007, India
2Thalassemia Control cell, Hindu Rao Hospital, Delhi, India
3Department of Pediatrics, Hindu Rao Hospital, Delhi, India

Received 13 April 2014; Accepted 16 July 2014; Published 12 October 2014

Academic Editor: Josep Esteve-Romero

Copyright © 2014 Leela Pant et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Thalassemia and thalassemic hemoglobinopathies pose serious health problem leading to severe morbidity and mortality in Indian population. Plethora of hemoglobin variants is prevalent in multiethnic Indian population. The aim of the present study was to analyze laboratory aspects, namely, hematological profile and HPLC findings of the hemoglobin variants detected, and to discuss problems that we faced in diagnosis in a routine clinical laboratory. We screened a total of 4800 cases in a hospital based population of North India in a 2-years period of by automated HPLC method using the Variant Hemoglobin Testing System (Variant II Beta Thalassemia Short Program, Bio-Rad Laboratories) under the experimental conditions specified by the manufacturer. Whole blood in EDTA was used and red cell indices were determined using automated hematology analyzer. We detected 290 cases with abnormal variants in which beta thalassemia was the most common followed by hemoglobin E. Here, we discuss the laboratory aspects of various hemoglobin disorders and diagnostic difficulties in cases like borderline HbA2 values, presence of silent mutation, alpha thalassemia gene, and few rare variants which at times require correlation with genetic study. Special attention was given to HbA2 level even in presence of a structural variant to rule out coinheritance of beta thalassemia gene.