Table of Contents
International Scholarly Research Notices
Volume 2014, Article ID 278173, 5 pages
http://dx.doi.org/10.1155/2014/278173
Research Article

Development and Validation of Stability Indicating Spectroscopic Method for Content Analysis of Ceftriaxone Sodium in Pharmaceuticals

1Department of Pharmaceutical Analysis, The Erode College of Pharmacy, Erode, Tamil Nadu 638112, India
2Department of Pharmaceutics, The Erode College of Pharmacy, Erode, Tamil Nadu 638112, India

Received 10 March 2014; Accepted 14 May 2014; Published 24 August 2014

Academic Editor: Jose Vicente Garcia Mateo

Copyright © 2014 Revathi Ethiraj et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A simple, selective, and stability indicating spectroscopic method has been selected and validated for the assay of ceftriaxone sodium in the powder for injection dosage forms. Proposed method is based on the measurement of absorbance of ceftriaxone sodium in aqueous medium at 241 nm. The method obeys Beer’s law in the range of 5–50 μg/mL with correlation coefficient of 0.9983. Apparent molar absorptivity and Sandell’s sensitivity were found to be L mol−1 cm−1 and 0.02732 μg/cm2/0.001 absorbance units. This study indicated that ceftriaxone sodium was degraded in acid medium and also underwent oxidative degradation. Percent relative standard deviation associated with all the validation parameters was less than 2, showing compliance with acceptance criteria of Q2 (R1), International Conference on Harmonization (2005) guidelines. Then the proposed method was successfully applied to the determination of ceftriaxone sodium in sterile preparation and results were comparable with reported methods.