Rapid Tests versus ELISA for Screening of HIV Infection: Our Experience from a Voluntary Counselling and Testing Facility of a Tertiary Care Centre in North India

Table 1

Characteristics of human immunodeficiency virus (HIV) tests as per kit literatures.

Test	Principle	Specimen volume required	Antigen employed	Time to test result	Specimen type	Sensitivity	Specificity
SD Bioline HIV-1/2 3.0	Lateral flow immunochromatography	10 𝜇L	gp 41 and p24 for HIV-1 and gp 36 for HIV-2	5–20; minutes	Serum,; plasma	Not; specified	Not; specified
Pareekshak HIV-1/2 Triline card test	Lateral flow immunochromatography	10 𝜇L	gp 41 and C terminus of gp 120 for HIV-1 and gp 36 for HIV-2	20; minutes	Serum,; plasma	100%	99.49%
Pareekshak HIV 1/2 rapid test kit (Trispot)	Immunoconcentration	2 drops	HIV 1 and HIV-2 r (recombinant)-proteins	Immediate	Serum,; plasma	100%	100%
Microlisa-HIV	Indirect ELISA	10–20 𝜇L	gp 41 and C terminus of gp 120 for HIV-1 and gp 36 for HIV-2	>1.5; hours	Serum,; plasma	100%	99.5%

HIV: human immunodeficiency virus; ELISA: enzyme linked immunosorbent assay.