Microplate Agglutination Test for Canine Brucellosis Using Recombinant Antigen-Coated Beads

Figure 1

Western blot analysis of recombinant antigens. TF fusions with Hsp60, RBP, SOD, and Ag 4 were separated by SDS-PAGE under reducing condition and then transferred to nylon membranes. The membranes were stained with Coomassie brilliant blue (CBB) and were used for analysis of B. canis-infected (WB, positive) and B.canis-uninfected sera (WB, negative).