Development and Validation of High Performance Liquid Chromatography Method for Simultaneous Estimation of Flavonoid Glycosides in Withania somnifera Aerial Parts
Table 2
Results of validation of HPLC method.
Validation parameters
1
2
3
Specificity
Peak purity (%)
>98
>98
>98
Linearity
Conc. range, µg/mL
8.82 to 564.30
8.55 to 547.20
9.11 to 583.30
0.9996
0.9996
0.9995
LOD
µg/mL
0.5
0.5
0.5
LOQ
µg/mL
1.5
1.5
1.5
Precision
Interday precision
(RSD), %
1.45
1.58
1.22
Intraday precision
(RSD), %
1.38
1.55
2.10
Accuracy: at three different concentrations in triplicate ()
