Table of Contents
International Scholarly Research Notices
Volume 2014 (2014), Article ID 391692, 9 pages
http://dx.doi.org/10.1155/2014/391692
Research Article

In Vitro and In Vivo Biochemical Evaluations of the Methanolic Leaf Extract of Garcinia kola

1Department of Biochemistry, Ladoke Akintola University of Technology, PMB 4000, Ogbomosho 210211, Nigeria
2Department of Medical Biosciences, University of the Western Cape, Private Bag X17, Bellville 7535, South Africa

Received 27 May 2014; Accepted 3 September 2014; Published 30 October 2014

Academic Editor: Graziella Vecchio

Copyright © 2014 Jelili A. Badmus et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Garcinia kola Heckel (Guttiferae) leaves have received limited scientific attention despite their traditionally acclaimed medicinal properties. The scavenging ability of the methanolic leaf extract (MLE) of G. kola was assayed for hydroxyl radical (OH), superoxide anion (), 1,1-diphenyl-2-picrylhydrazyl (DPPH), azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS•+), and lipid peroxidation (LP) activity in egg yolk, rat liver, and brain homogenates. Total phenolic and flavonoid contents of the extract were also evaluated. Group I animals were given oral doses of water, whereas Group II and Group III animals received 100 and 200 mg/kg body weight (bw) MLE, respectively, for 14 days. Plasma glucose, magnesium, -glutamyltransferase (GGT/GT), alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine, and urea were evaluated. Hepatic reduced glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD), LP, and liver histopathological appearance were also assessed. The extract scavenged OH, , DPPH, and ABTS•+ and inhibited LP in egg yolk, rat liver, and brain homogenates. Furthermore, oral administration of the extract showed no adverse effects on hepatic and renal function tests. Increased hepatic GSH and nonsignificant changes in LP, GPx and SOD activities, and liver histology were observed. These results suggest that G. kola leaves have antioxidant activities which may have application in traditional medicine.