Synthesis of Novel Pyridopyridazin-3(2H)-one Derivatives and Evaluation of Their Cytotoxic Activity against MCF-7 Cells

Table 1

Inhibitory effects of compounds 2a–f, 3a–f on viability of breast cancer cells.

Compounds	R	MCF-7 IC50^a (𝜇M)
2a	H	475.4±27.8
2b	CH3	498.4±30.0
2c	OCH3	344.1±59.4
2d	Cl	61.3±5.8
2e	Br	78.9±14.7
2f	3-NO2	141.0±22.5
3a	H	268.7±15.4
3b	CH3	219.0±11.0
3c	OCH3	248.1±17.2
3d	Cl	364.1±20.2
3e	Br	810.0±35.7
3f	3-NO2	473.7±26.1
Doxorubicin^b	—	2.007±0.05

aInhibitory concentration (IC₅₀, 𝜇M) as obtained from MTT assay. All the values represent the average of three experimental samples. The cells were incubated for a period of 48 h.
bDoxorubicin was used as the standard drug.