Table of Contents
International Scholarly Research Notices
Volume 2014, Article ID 429828, 10 pages
http://dx.doi.org/10.1155/2014/429828
Research Article

Adaptogenic Activity of Lyophilized Hydroethanol Extract of Pandanus odoratissimus in Swiss Albino Mice

1Post Graduates Department of Pharmacology, JSPM’s Jayawantrao Sawant College of Pharmacy and Research, University of Pune, Pune, Maharashtra 411028, India
2Vinayaka Missions University, Sankari Main Road, NH-47, Ariyanoor, Salem, Tamil Nadu 636308, India
3Department of Pharmaceutical Medicinal Chemistry, Gahlot Institute of Pharmacy, Plot No. 59, Sector No. 14, Koparkhairane, Navi-Mumbai, Maharashtra 400709, India
4JSPM’s Charak College of Pharmacy and Research, University of Pune, Pune, Maharashtra 412207, India

Received 16 April 2014; Revised 16 July 2014; Accepted 6 August 2014; Published 29 October 2014

Academic Editor: Federica Pellati

Copyright © 2014 Prafulla P. Adkar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. The leaves of Pandanus odoratissimus Linn have been widely used in Ayurveda to treat a variety of common and stress related disorders. In the present investigation, hydroethanol extract of leaves of Pandanus odoratissimus Linn (LEPO) were evaluated for antistress activity in normal and stress induced mice. Furthermore, the extract was studied for nootropic (adaptogenic) activity in mice and in vitro antioxidant potential to correlate with its adaptogenic and antistress activity. LEPO (100 and 200 mg/kg p.o) was evaluated against forced swimming endurance stress test, anoxia stress tolerance and immobilization stress and chronic cold resistant stress tests, and biomarkers (serum glucose, Corticosterone, WBC, RBC, and DLC count) to assess the antistress activity in mice. Withania somnifera (WS) (100 mg/kg p.o) was selected as reference standard. The parameters like anoxia stress tolerance time were recorded in anoxia stress and estimation of biochemical marker levels and determination of organs weight were carried out in immobilization stress models. Results. Concomitant treatment with LEPO 200 mg/kg significantly increased in anoxia stress tolerance time. Dose dependent significant reduction in serum glucose, corticosterone, and WBC, RBC, and DLC was observed in immobilisation stress model as compared to stressed group. LEOP 200 mg/kg and WS 100 mg/kg significantly reversed/inhibited the stress induced changes in these parameters. The results from the present study indicate that these values also express that dose dependent significant adaptogenic activity in stressed animals. Conclusion. The present study provides scientific support for the antistress (adaptogenic) and nootropic activities of lyophilized hydroethanol extract of Pandanus odoratissimus Linn and substantiate the traditional claims for the usage of Pandanus in stress induced disorders.