Detection and Assay of Vitamin B-2 (Riboflavin) in Alkaline Borate Buffer with UV/Visible Spectrophotometry
Figure 2
Absorbance spectrum of riboflavin at various pH and buffer. (a) is pH 7.36 utilizing phosphate-saline buffer (phosphate 6.6 millimolar and saline 0.154 molar) with riboflavin at 1.403 × 10−5 molar. (b) is pH 7.52 utilizing sodium borate buffer at 0.010 molar and riboflavin at 1.403 × 10−5 molar. (c) is pH 9.01 utilizing Tris buffer (25 millimolar Tris base and 150 millimolar glycine) with riboflavin at 1.403 × 10−5 molar.
