Identification of Unique miRNA Biomarkers in Colorectal Adenoma and Carcinoma Using Microarray: Evaluation of Their Putative Role in Disease Progression
Figure 3
(a) We compared 2406 genes in adenoma and 1403 genes in carcinoma to find the regulatory genes controlled by miRNAs that potentially mediate the carcinogenesis in adenoma-carcinoma sequel. (A) miR-33 is unique to colon adenoma stage and is responsible for the suppression of gamma-aminobutyric acid receptor beta-subunit gene (GABRB2). (B) The other miRNA which was differentially expressed was miR-147. We identified the matching partners for miR-147 by comparing the same gene sets used for miR-135b. Interestingly, both miR-147 and miR-135a/miR-135b were not expressed in colon adenoma; however, they are both upregulated in colon carcinoma. (b) Graphical representation of adenoma-carcinoma sequel starting from normal colon and specific miRNAs and gene (oncogenes and tumor suppressors) associated with the sequel. ΩTumor suppressor, #oncogene, †oncomirs, and *mature miRNA sequence from the opposite arm of the precursor.
