Crotalus durissus collilineatus Venom Induces TNF-α and IL-10 Production in Human Peripheral Blood Mononuclear Cells
Figure 3
Expression of activation (CD69) and regulation (PD1) markers. PBMCs were cultured in presence of Cdt and Cdc venom. After 24 h, cells were stained with antibodies for CD8, PD1, and CD69 and analyzed by flow cytometry. A gate was done in the region of lymphocytes and those cells were gated as CD8+ and CD8− subsets and the expression of PD1 and CD69 was analyzed inside of these subsets. (a) CD69 expression on CD8+ T cells. (b) PD1 expression on CD8+ T cells. (c) CD69 expression on CD8− T cells. (d) PD1 expression on CD8− T cells. The analysis was done using Mann-Whitney test and the horizontal bars show the median. Cdt: Crotalus durissus terrificus venom; Cdc: Crotalus durissus collilineatus venom; NS: nonstimulated cells.
