Development and Validation of HPTLC Method for Simultaneous Estimation of Diosgenin and Quercetin in Fenugreek Seeds (Trigonella foenum-graceum)

Table 1

Method validation parameters for the quantification of diosgenin and quercetin by proposed HPTLC method.

Validation parameters	Results for diosgenin	Results for quercetin
Regression equation	𝑌=3416+27.27𝑋	𝑌=1055+10.81𝑋
Correlation coefficient 𝑛=3	0.990	0.985
Linearity range (ng/spot), 𝑛=3	100–500	100–1000
𝑅𝑓	0.69±0.02	0.57±0.02
Limit of detection (ng/spot), 𝑛=3	12.52	15.3
Limit of quantification (ng/spot), 𝑛=3	45	40
Instrumental precision (RSD), 𝑛=6	0.42	0.56
Method precision (RSD) 𝑛=6	0.61	0.67
Specificity	Specific	Specific
Robustness (RSD), 𝑛=3	0.83	0.94