Development and Validation of HPTLC Method for Simultaneous Estimation of Diosgenin and Quercetin in Fenugreek Seeds (Trigonella foenum-graceum)

Table 2

Results from study of recovery for diosgenin and quercetin.

Compound	Amount of compound present in plant material (𝜇g/g)	Amount of standard added (ng)	Amount of standard found in mixture (𝜇g)	Recovery^#	Average recovery^# (%)
Quercetin	354	391.3	742.5±0.62	99.62±0.60	99.63±0.34
	354	568.8	918.7±0.17	99.55±0.59
	354	643.4	994.8±0.48	99.73±0.48
Diosgenin	402	327.23	721.08±0.34	98.88±0.33	99.13±0.26
	402	467.12	865.05±0.23	99.53±0.22
	402	587.02	979.10±0.17	98.99±0.16

#Mean ± relative standard deviation (%); 𝑛=3.