Table of Contents
International Scholarly Research Notices
Volume 2014 (2014), Article ID 583730, 9 pages
http://dx.doi.org/10.1155/2014/583730
Research Article

Hypertrophic Scar Formation on Application of Terpenoid Fraction of Tuberous Root of Mirabilis jalapa L. on Excision Wound Model in Wistar Albino Rats

1Defence Research Laboratory, Post Bag No. 2, Tezpur, Assam 784001, India
2Department of Life Sciences, Dibrugarh University, Dibrugarh, Assam 786004, India

Received 26 May 2014; Revised 15 September 2014; Accepted 18 September 2014; Published 13 November 2014

Academic Editor: Giuseppe Maurizio Campo

Copyright © 2014 Jyotchna Gogoi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The study was designed to evaluate the effects of hydromethanolic extract of tuberous root of M. jalapa and its terpenoid and flavonoid fractions on cutaneous wound healing in Wistar Albino rats. The hydromethanolic extract was subfractionated by sequential extraction in solvents (moderately nonpolar to polar). The extract and its (terpenoid and flavonoid) fractions were used for cutaneous wound healing studies by using excision wound model on rat. Their effects on wound contraction rate, biochemical and histological changes, and expression of growth factors such as collagen 3A, basic fibroblast growth factor, and vascular endothelial growth factor were investigated. The results indicated that flavonoid treated group showed significant decrease () in antioxidant enzyme level as compared to control in wound healing process, whereas terpenoid fraction showed significant increase () in expression of growth factor levels but regeneration and remodeling stages were delayed due to formation of thicker ulcus layer and also there were no hair follicle-like blood capillaries formation which ultimately may lead to formation of hypertrophic scar of wound. Therefore, from this study, it can be concluded that terpenoid fraction prolongs proliferation phase and hence may have tendency to convert the wound into hypertrophic wound.