Use of Nonspecific, Glutamic Acid-Free, Media and High Glycerol or High Amylase as Inducing Parameters for Screening Bacillus Isolates Having High Yield of Polyglutamic Acid
SDS-PAGE of EPS from Bacillus K. SDS-PAGE of native and hydrolysed EPS, using 5% stacking and 10% resolving gel and detection by staining with coomassie brilliant blue (CBB) or methylene blue (MB) or silver stain (SS). Stacking gel portion was retained for lanes 1, 2, and 3. Lanes: 1 = bovine serum albumin; 100 microgram, 2,4 = 1000 microgram. 5 = 10 microgram; 3 = acid-hydrolysed polymer.