Isocratic High-Performance Liquid Chromatographic Assay of Olanzapine: Method Development and Validation

Table 2

Results of robustness study (OLP concentration, 10 μg mL⁻¹).

Chromatographic condition	Alteration	Peak area precision			Retention time precision
		Mean area* ± SD	SE	RSD, %	Mean 𝑅𝑇^∗ ± SD	SE	RSD, %
Mobile phase flow rate (mL min^−1)	0.9	606358 ± 0.0058	0.003	0.052	4.819 ± 0.004	0.023	0.073
	1.0	547577 ± 0.000	0.000	0.000	4.364 ± 0.003	0.002	0.070
	1.1	498323 ± 0.010	0.006	0.108	3.972 ± 0.002	0.002	0.038
Column temperature (°C)	38	546292 ± 0.025	0.014	0.249	4.399 ± 0.020	0.011	0.464
	40	548214 ± 0.006	0.003	0.057	4.364 ± 0.001	0.006	0.023
	42	548214 ± 0.020	0.012	0.198	3.972 ± 0.002	0.006	0.023

∗Mean value of three injections.