Efficient Electrochemical <i>N</i>-Alkylation of <i>N</i>-Boc-Protected 4-Aminopyridines: Towards New Biologically Active Compounds

Feroci, Marta; Chiarotto, Isabella; Forte, Gianpiero; Simonetti, Giovanna; D'Auria, Felicia Diodata; Maes, Louis; De Vita, Daniela; Scipione, Luigi; Friggeri, Laura; Di Santo, Roberto; Tortorella, Silvano

doi:https://doi.org/10.1155/2014/621592

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Research Article | Open Access

Volume 2014 | Article ID 621592 | https://doi.org/10.1155/2014/621592

Efficient Electrochemical N-Alkylation of N-Boc-Protected 4-Aminopyridines: Towards New Biologically Active Compounds

Marta Feroci,¹Isabella Chiarotto,¹Gianpiero Forte,¹Giovanna Simonetti,²Felicia Diodata D'Auria,²Louis Maes,³Daniela De Vita,⁴Luigi Scipione,⁴Laura Friggeri,⁴Roberto Di Santo,⁴and Silvano Tortorella⁴

Academic Editor: R. Pohl, L. Palombi

Received22 Nov 2013

Accepted21 Jan 2014

Published05 Mar 2014

Abstract

The use of electrogenerated acetonitrile anion allows the alkylation of N-Boc-4-aminopyridine in very high yields, under mild conditions and without by-products. The high reactivity of this base is due to its large tetraethylammonium counterion, which leaves the acetonitrile anion “naked.” The deprotection of the obtained compounds led to high yields in N-alkylated 4-aminopyridines. Nonsymmetrically dialkylated 4-aminopyridines were obtained by subsequent reaction of monoalkylated ones with t-BuOK and alkyl halides, while symmetrically dialkylated 4-aminopyridines were obtained by direct reaction of 4-aminopyridine with an excess of t-BuOK and alkyl halides. Some mono- and dialkyl-4-aminopyridines were selected to evaluate antifungal and antiprotozoal activity; the dialkylated 4-aminopyridines 3ac, 3ae and 3ff showed antifungal towards Cryptococcus neoformans; whereas 3cc, 3ee and 3ff showed antiprotozoal activity towards Leishmania infantum and Plasmodium falciparum.

1. Introduction

N-Alkylated 4-aminopyridine is a common moiety in biologically active molecules. It is present, in fact, in compounds with different activities such as inhibitors of p38 MAP kinase [1], inhibitors of HIV-EP1 cellular transcription factor [2], inhibitors of coagulation Factor Xa [3], and -chemokine receptor CCR5 antagonists in anti-HIV therapy [4]; in particular we have focused our work on the development of new CYP51 inhibitors, active both on fungal strains [5] and Trypanosoma Cruzi [6]. Many literature data evidenced that the pyridine group can efficaciously replace the heme-iron chelating azole moiety present in classical azole CYP51 inhibitors and, therefore, the alkylation of 4-aminopyridine (4AP) represents an important goal in organic synthesis to develop novel classes of antifungal and antiparasitic drugs [7, 8].

Due to the wide presence of these products, the alkylation of 4-aminopyridine (4AP) is therefore an important goal in organic synthesis.

Different approaches to obtain N-alkylated 4-aminopyridines have been reported in the literature. Some examples are the efficient condensation of 4AP with alcohols catalyzed by benzaldehyde [9] or copper [10, 11] or magnetite [12], the reaction of 4AP with an acyl chloride, and the following reduction of the amide with LiAlH₄ [13].

The most straightforward method, however, is the direct alkylation of 4AP with alkyl halides, although it suffers from some drawbacks. The two different nitrogen atoms compete in the alkylation reaction and usually the more nucleophilic pyridine nitrogen atom reacts faster, leading to the corresponding pyridinium salt (Scheme 1) [14, 15].

In these case, the use of a very strong base is therefore necessary: n-BuLi was successfully used by Singh and coworkers [16], obtaining N-methyl- and N-ethyl-4-aminopyridines in 74–80% yields.

A viable alternative is the enhancement of the nucleophilicity of the amine nitrogen atom (versus the pyridine one), allowing the use of weaker bases. An example is the activation of 2-aminopyridine as formyl or Boc derivative at the amine nitrogen atom [17], with subsequent deprotonation using sodium hydride, alkylation, and deprotection with trifluoroacetic acid. The deprotonation of N-Boc-2-aminopyridine with NaH needs a careful control of the temperature (0–5°C) and is carried out in anhydrous DMF, with a vigorous stirring required to keep the suspension fluid.

In this context, we envisaged the possibility to alkylate N-Boc-4-aminopyridine (N-Boc-4AP) using milder reaction conditions, that is, using electrogenerated tetraethylammonium cyanomethanide (Et4N^+-CH₂CN) [18]. This base, the acetonitrile anion, can be easily obtained by cathodic galvanostatic reduction of a solution of acetonitrile containing tetraethylammonium hexafluorophosphate as supporting electrolyte (Scheme 2), without by-products (the reagent is the electron), and it was successfully used by us in a good variety of reactions [19], such as the selective N-alkylation of bifunctional compounds [20], the Gewald reaction [21], the synthesis of -lactams [22], and the synthesis of carbamates [23]. The actual mechanism for the formation of acetonitrile anion is not known, but a hypothesis based on the direct reduction of the tetraethylammonium cation has been reported (Scheme 2) [24].

The high reactivity of this base is ascribable to the large tetraethylammonium counterion, which renders the acetonitrile anion extremely reactive. Moreover, its reaction as a base gives no by-products, as the protonation restores the molecule of solvent.

2. Results and Discussion

The reaction of electrogenerated acetonitrile anion with 4AP, followed by an alkyl halide, leads to poor yields in desired compound with the pyridinium salt being the major product. This prevents the direct use of ⁻CH₂CN with 4AP. On the other hand, if the amine nitrogen is activated as Boc derivative (N-Boc-4AP), the deprotonation/alkylation reaction using acetonitrile anion leads to products1 in very high yields (Scheme 3 and Table 1, entries 1–6). The classic deprotection with trifluoroacetic acid allows obtaining the desired products2 (Scheme 3 and Table 1, entries 1–6).

The data in Table 1 highlight that the reaction of deprotonation of N-Boc-4AP using electrogenerated acetonitrile anion, alkylation with both alkyl and benzyl halides, and deprotection with trifluoroacetic acid is very efficient, with overall yields of 78–86%. However, when the alkylating agent is a bromoacetophenone, the yields in alkylated product are lower and in most cases the deprotection reaction leads to the dealkylation of the starting material (Table 1, entries 7–10).

As many biologically active compounds contain the dialkylated 4-aminopyridine moiety, we tried to carry out a second alkylation on products 2a–j using acetonitrile anion but, as expected, the high nucleophilicity of the pyridine nitrogen led to the synthesis of the corresponding pyridinium salt.

We thus carried out this second alkylation using strong bases, the most efficient being t-BuOK in DMSO (Scheme 4), although the yields in dialkylated 4AP were not very high. The results of this reaction are reported in Table 2.

In order to obtain symmetrically dialkylated 4AP, 4AP was subjected to deprotonation with t-BuOK in DMSO, adding an excess of alkylating agent. The reaction led to a mixture of mono- and dialkylated 4-aminopyridines, in moderate to acceptable yields. The results are reported in Table 3.

3. Biological Activity

A selection of synthesized compounds was in vitro tested to evaluate antifungal activity against different strains of C. albicans, C. parapsilosis, and Cryptococcus neoformans; data are reported in Table 4. As can be evidenced the nonsymmetrical dialkylated 4APs 3ac and 3ae showed a moderate antifungal activity towards C. albicans and C. parapsilosis with MIC₅₀ values of 32 μg/mL and showed an interesting activity against Cryptococcus neoformans, with MIC₅₀ values of 0.4 and 4 μg/mL, respectively. Otherwise, the symmetrical dialkylated 4APs 3cc, 3ee and the Boc-protected monoalkylated 4APs 1b, 1e, 1f showed poor antifungal activity with MIC₅₀ and MIC₁₀₀ ≥ 64 μg/mL.

Furthermore, the symmetrical dialkylated 4APs 3cc, 3ee, and 3ff were in vitro tested to evaluate the activity against Trypanosoma cruzi, Trypanosoma brucei, Leishmania infantum, and Plasmodium falciparum; the results are summarized in Table 5.

As can be evidenced, all tested compounds showed a moderate activity versus P. falciparum and an interesting activity towards L. infantum with IC₅₀ values lower than the reference drug miltefosine; otherwise, they resulted scarcely active against T. cruzi and T. brucei. Moreover, these compounds also showed low toxic activity versus growing MRC-5 cells.

4. Conclusion

In conclusion, we demonstrated the usefulness of electrogenerated acetonitrile anion in the alkylation of N-Boc 4-aminopyridines, both from the point of view of the high yields and of the cleanliness of the reaction (no by-products). The deprotection of N-Boc 4-aminopyridines allowed obtaining monoalkylated 4-aminopyridine in very high yields. The following alkylation, by means of t-BuOK and alkyl halides, led to nonsymmetrically dialkylated 4-aminopyridine, while symmetrically dialkylated products were obtained directly from 4-aminopyridine by reaction with an excess of t-BuOK and alkyl halide.

Furthermore, it can also be concluded that the monoalkylation of the 4AP leads to inactive products and otherwise interesting activity against fungi and some protozoa can be obtained by dual, symmetrical, or nonsymmetrical dialkylation of the amino group of 4AP; these active molecules can be considered as lead compound to develop new antifungal and antiprotozoal compounds.

5. Materials and Methods

5.1. General

Acetonitrile was distilled twice from P₂O₅ and CaH₂. Commercially available reagents were used without further purification. The Boc protection of 4-aminopyridine was carried out following the literature [25].

4-[N-(tert-Butoxycarbonyl)amino]pyridine N-Boc-4AP. To a solution of di-tert-butyl dicarbonate (3 mmol) in acetonitrile (3 cm³) at room temperature 4-aminopyridine (3 mmol) was slowly added. This mixture was then allowed to stir for 3 h at room temperature. The solvent was evaporated and the crude 4-[N-(tert-butoxycarbonyl)amino]pyridine (>95%) was used in the electrolyses without further purification. R_f (30% ethyl acetate in light petroleum ether) 0.20; ¹H NMR (200 MHz, CDCl₃) 1.53 (s,9H), 6.9 (bs, 1H), 7.32 (dd,, 1.6 Hz, 2H), 8.45 (dd,, 1.6 Hz, 2H); ¹³C NMR (50 MHz, CDCl₃) 28.2, 81.7, 112.3, 145.6, 150.3, 151.9; EIMS, m/z: 194 (M^.+, 1%), 137 (2%), 121 (5%), 120 (8%), 94 (50%), 78 (4%), 57 (100%).

5.2. Electrochemical ALkylation of N-Boc-4AP

Constant current electrolyses (I = 25 mA cm⁻²) were performed under a nitrogen atmosphere, at 20°C, using an Amel Model 552 potentiostat equipped with an Amel Model 731 integrator. All the experiments were carried out in a divided glass cell separated through a porous glass plug filled up with a layer of gel (i.e., methyl cellulose 0.5% volume dissolved in DMF-Et₄NPF₆ 1.0 mol dm⁻³); Pt spirals (apparent areas 0.8 cm²) were used both as cathode and anode. MeCN-Et₄NPF₆ 0.1 mol dm⁻³ was used as solvent-supporting electrolyte system (catholyte: 20 cm³; anolyte: 5 cm³). 1 mmol of N-Boc-4-aminopyridine was present in the catholyte. After 145 C were passed, the current was switched off and 1 mmol of alkylating agent was added to the catholyte. The solution was kept under stirring at room temperature for 2 hours; then the solvent was evaporated under reduced pressure and the residue was purified by flash column chromatography, using a mixture of ethyl acetate/light petroleum ether 2/8 in volume, obtaining the pure products.

Flash column chromatography was carried out using Merck 60 kieselgel (230–400 mesh) under pressure. GC-MS measurements were carried out on SE 54 capillary column using a Fisons 8000 gas chromatograph coupled with a Fisons MD 800 quadrupole mass selective detector. ¹H and ¹³C NMR spectra were recorded at room temperature using a Bruker AC 200 spectrometer using CDCl₃ as internal standard.

tert-Butyl (octyl)pyridin-4-ylcarbamate 1a. R_f (80% ethyl acetate in dichloromethane) 0.60; ¹H NMR (200 MHz, CDCl₃) δ 0.88 (), 1.20–1.30 (m, 10H), 1.49–1.86 (m, 3H), 1.50 (s, 9H), 3.69 (), 7.24 (dd,, 1.6 Hz, 2H), 8.51 (); ¹³C NMR (50 MHz, CDCl₃) 14.0, 22.6, 26.7, 28.2, 28.4, 29.1, 31.7, 48.7, 81.4, 118.8, 150.0, 150.1, 153.4.

tert-Butyl (3-phenylpropyl)pyridin-4-ylcarbamate 1b. R_f (50% ethyl acetate in light petroleum ether) 0.46; ¹H NMR (200 MHz, CDCl₃) 1.48 (s, 9H), 1.86–2.02 (m, 2H), 2.64 (t Hz, 2H), 3.74 (), 7.12–7.33 (m, 7H), 8.49 (); ¹³C NMR (50 MHz, CDCl₃) 28.2, 30.0, 33.0, 48.3, 81.6, 118.9, 126.1, 128.3, 128.5, 141.0, 149.7, 150.3, 153.4; EIMS, m/z: M^.+ absent, 212 (5%), 107 (100%), 105 (5%), 91 (25%), 78 (27%), 77 (12%).

tert-Butyl (benzyl)pyridin-4-ylcarbamate 1c. R_f (60% ethyl acetate in light petroleum ether) 0.58; ¹H NMR (200 MHz, CDCl₃) 1.45 (s, 9H), 4.94 (s, 2H), 7.20–7.37 (m, 7H), 8.46 (dd,, 1.6 Hz, 2H); ¹³C NMR (50 MHz, CDCl₃) 28.1, 52.5, 82.1, 118.2, 126.3, 127.3, 128.7, 137.7, 150.2, 150.1, 153.5; EIMS, m/z: M^.+ absent, 227 (4%), 183 (14%), 91 (100%), 78 (7%), 57 (51%).

tert-Butyl (2,6-dichlorobenzyl)pyridin-4-ylcarbamate 1d. R_f (50% ethyl acetate in light petroleum ether) 0.60; ¹H NMR (200 MHz, CDCl₃) 1.48 (s, 9H), 5.29 (s, 2H), 7.02–7.21 (m, 5H), 8.41 (); ¹³C NMR (50 MHz, CDCl₃) 28.2, 46.7, 81.7, 121.5, 128.6, 129.5, 131.7, 136.1, 148.1, 149.9, 153.3; EIMS, m/z: 352 (M^.+, 1%), 252 (3%), 163 (6%), 161 (30%), 159 (42%), 78 (76%), 51 (100%).

tert-Butyl (4-fluorobenzyl)pyridin-4-ylcarbamate 1e. R_f (50% ethyl acetate in light petroleum ether) 0.49; ¹H NMR (200 MHz, CDCl₃) δ 1.45 (s, 9H), 4.89 (s, 2H), 6.97–7.22 (m, 6H), 8.47 (app dHz, 2H); ¹³C NMR (50 MHz, CDCl₃) δ 28.1, 51.8, 82.2, 116.6 (d, Hz), 118.4, 128.1 (d, Hz), 133.4 (d, Hz), 149.9, 150.3, 153.4, 162.2 (d,Hz); EIMS, m/z: 302 (M^.+, 1%), 245 (4%), 201 (53%), 108 (100%), 78 (42%), 57 (100%).

tert-Butyl (4-trifluoromethylbenzyl)pyridin-4-ylcarbamate 1f. R_f (50% ethyl acetate in light petroleum ether) 0.41; ¹H NMR (200 MHz, CDCl₃) 1.46 (s, 9H), 4.99 (s, 2H), 7.20–7.36 (m, 4H), 7.61 (app d, Hz, 2H), 8.49 (app d Hz, 2H); ¹³C NMR (50 MHz, CDCl₃) 28.1, 52.2, 82.5, 118.1, 124.0 (q, Hz), 125.7 (q, Hz), 126.6, 129.7 (q, Hz), 141.9, 149.8, 150.4, 153.3; EIMS, m/z: M^.+ absent, 251 (9%), 158 (34%), 145 (2%), 78 (25%), 69 (9%), 57 (100%).

tert-Butyl (2-oxo-2-phenylethyl)pyridin-4-ylcarbamate 1 g. R_f (60% ethyl acetate in light petroleum ether) 0.50; ¹H NMR (200 MHz, CDCl₃) δ 1.46 (s, 9H), 5.09 (s, 2H), 7.26 (d, Hz, 2H), 7.48–7.68 (m, 3H), 7.99 (d, Hz, 2H), 8.47–8.55 (m, 2H); ¹³C NMR (50 MHz, CDCl₃) 28.1, 55.5, 82.5, 118.7, 127.9, 128.9, 133.9, 134.7, 150.2, 153.2, 193.7.

tert-Butyl (2-(4-fluoropheny)-2oxoethyl)pyridin-4-ylcarbamate 1 h. R_f (50% ethyl acetate in light petroleum ether) 0.50; ¹H NMR (200 MHz, CDCl₃) δ 1.46 (s, 9H), 5.05 (s, 2H), 7.15–7.27 (m, 4H), 7.99–8.06 (m, 2H), 8.52 (dd, J = 5.0, 1.4 Hz, 2H); ¹³C NMR (50 MHz, CDCl₃) 28.1, 55.4, 82.6, 116.2 (d, Hz), 118.8, 130.6 (d, Hz), 131.1 (d, Hz), 150.1, 150.2, 153.2, 166.2 (d, Hz), 192.2.

tert-Butyl (2-(4-chloropheny)-2oxoethyl)pyridin-4-ylcarbamate 1i. R_f (20% ethyl acetate in dichloromethane) 0.30; ¹H NMR (200 MHz, CDCl₃) δ 1.45 (s, 9H), 5.04 (s, 2H), 7.21 (dd, Hz, 2H), 7.50 (d, Hz, 2H), 7.93 (d, Hz, 2H), 8.51 (dd, Hz, 2H); ¹³C NMR (50 MHz, CDCl₃) 28.1, 55.4, 82.6, 118.8, 129.3, 133.0, 140.5, 149.9, 150.3, 153.1, 192.7.

tert-Butyl (2-(4-methoxypheny)-2oxoethyl)pyridin-4-ylcarbamate 1j. R_f (40% ethyl acetate in dichloromethane) 0.50; ¹H NMR (200 MHz, CDCl₃) δ 1.46 (s, 9H), 3.90 (s, 3H), 5.04 (s, 2H), 6.99 (d, Hz, 2H), 7.24 (dd, Hz, 2H), 7.97 (d, Hz, 2H), 8.50 (dd,, 1.6 Hz, 2H); ¹³C NMR (50 MHz, CDCl₃) 28.1, 55.1, 55.5, 82.3, 114.1, 118.7, 127.8, 130.2, 150.2, 153.3, 164.1, 192.0.

5.3. Deprotection of Compounds 1a–j

To a solution of 1 (1 mmol) in CH₂Cl₂ (5 cm³), kept at 0°C, 1 cm³ of CF₃COOH was added. This mixture was allowed to stir for 3 h at 0°C. The solution was then mixed with aqueous sodium carbonate till pH 8 and extracted with ethyl acetate. The solvent was removed under reduced pressure and the mixture was purified by flash chromatography, yielding pure compound 2.

N-(Octyl)pyridin-4-amine 2a. R_f (20% dichloromethane in ethyl acetate) 0.16; ¹H NMR (200 MHz, CDCl₃) δ 0.89 (t, Hz, 3H), 1.25–1.34 (6H), 1.61–1.70 (m, 2H), 2.89–3.12 (m, 2H), 3.15–3.24 (m, 2H), 5.43–5.48 (m, 2H), 6.57 (d, Hz, 2H), 8.11 (d, Hz, 2H); ¹³C NMR (50 MHz, CDCl₃) 14.1, 22.6, 27.0, 28.8, 29.2, 29.2, 31.8, 42.9, 107.4, 155.2.

N-(3-Phenylpropyl)pyridin-4-amine 2b. R_f (ethyl acetate) 0.46; ¹H NMR (200 MHz, CD₃CN) 1.91–2.06 (m, 2H), 2.71 (t, Hz, 2H), 3.17–3.27 (m, 2H), 4.9 (bs, 1H), 6.62–6.66 (m, 2H), 7.14–7.46 (m, 5H), 7.45–7.97 (m, 2H); ¹³C NMR (50 MHz, CD₃CN) 29.8, 32.5, 42.0, 107.3, 125.9, 128.3, 128.4, 141.0, 141.5, 157.8; EIMS, m/z: 212 (M^.+, 1%), 107 (100%), 91 (43%), 78 (13%).

N-(Benzyl)pyridin-4-amine 2c. R_f (ethyl acetate) 0.57; ¹H NMR (200 MHz, CDCl₃) 4.46 (d, Hz, 2H), 6.7 (bs, 2H), 7.1 (bs, 1H), 7.21–7.41 (m, 5H), 8.0 (bs, 2H); ¹³C NMR (50 MHz, CDCl₃) 46.8, 107.4, 127.2, 127.6, 128.8, 137.7, 148.6, 154.0; EIMS, m/z: 184 (M^.+, 15%), 183 (15%), 107 (5%), 91 (100%), 78 (16%).

N-(2,6-Dichlorobenzyl)pyridin-4-amine 2d. R_f (ethyl acetate) 0.38; ¹H NMR (200 MHz, CD₃CN) 4.59 (s, 2H), 6.0 (bs, 1H), 6.68 (dd,, 1.4 Hz, 2H), 7.27–7.47 (m, 3H), 8.41 (app d, Hz, 2H); ¹³C NMR (50 MHz, CD₃CN) 42.2, 107.4, 128.7, 130.4, 132.8, 136.0, 147.3, 154.6; EIMS, m/z: 256 (M^.+ + 4, 1%), 254 (M^.+ + 2, 7%), 252 (M^.+, 14%), 162 (10%), 160 (67%), 158 (100%), 78 (37%).

N-(4-Fluorobenzyl)pyridin-4-amine 2e. R_f (ethyl acetate) 0.27; ¹H NMR (200 MHz, CDCl₃) 4.38 (d, J = 5.4 Hz, 2H), 5.1 (bs, 1H), 6.5 (bs, 2H), 7.02–7.10 (m, 2H), 7.30–7.34 (m, 2H), 8.2 (bs, 2H); ¹³C NMR (50 MHz, CDCl₃) 46.3, 107.8, 115.8 (d, Hz), 129.0 (d, Hz), 133.1, 148.5, 153.8, 166.8 (d, Hz),; EIMS, m/z: 202 (M^.+, 5%), 107 (16%), 109 (100%), 78 (16%).

N-(4-Trifluoromethylbenzyl)pyridin-4-amine 2f. R_f (ethyl acetate) 0.25; ¹H NMR (200 MHz, CDCl₃) 4.51 (d, Hz, 2H), 6.2 (bs, 1H), 6.6 (bs, 2H), 7.54 (d, Hz, 2H), 7.67 (d, Hz, 2H), 8.1 (bs, 2H); ¹³C NMR (50 MHz, CDCl₃) 45.4, 107.8, 124.4 (q, Hz), 125.4 (q, Hz), 128.4 (q, J = 31.9 Hz), 127.7, 143.2, 146.7, 155.0; EIMS, m/z: 252 (M^.+, 80%), 183 (11%), 159 (100%), 107 (52%), 78 (31%).

1-(4-Methoxyphenyl)-2-(pyridin-4-ylamine)ethan-1-one 2 g. R_f (50% ethyl acetate in ethanol) 0.15; ¹H NMR (200 MHz, ) 3.90 (s, 3H), 4.57 (d, Hz, 2H), 4.6 (bs, 1H), 6.58 (d, J = 6.2 Hz, 2H), 6.98 (d, Hz, 2H), 7.98 (d, Hz, 2H), 8.19 (d, Hz, 2H); ¹³C NMR (50 MHz, ) 48.2, 55.6, 108.1, 114.1, 127.2, 130.2, 150.8, 153.6, 164.5, 191.6.

5.4. Alkylation of Compounds 2a,c,e

To a solution of 2 (1 mmol) in anhydrous DMSO (2 cm³), kept at rt under N₂, 1.5 mmol of t-BuOK was added. This mixture was allowed to stir for 20 min at rt; then 1 mmol of alkyl halide was added and the solution was kept under stirring at rt for 4 h. The solution was then mixed with water and extracted with dichloromethane. The solvent was removed under reduced pressure and the mixture was purified by flash chromatography, yielding pure compound 3.

N-Benzyl-N-octylpyridin-4-amine 3ac. R_f (80% ethyl acetate in ethanol) 0.38; ¹H NMR (200 MHz, CDCl₃) 0.86–0.92 (m, 3H), 1.20–1.40 (m, 10H), 1.63–1.70 (m, 2H), 3.42 (app t, Hz, 2H), 4.59 (s, 2H), 6.51 (d, Hz, 2H), 7.14–7.38 (m, 5H), 8.18 (d, Hz, 2H); ¹³C NMR (50 MHz, CDCl₃) 14.1, 22.6, 26.9, 27.0, 29.2, 29.4, 29.7, 31.8, 50.7, 53.4, 106.9, 126.2, 127.3, 128.8, 136.8, 148.9, 153.6.

N-(4-Fluorobenzyl)-N-octylpyridin-4-amine 3ae. R_f (ethyl acetate) 0.40; ¹H NMR (200 MHz, CDCl₃) 0.86–0.92 (m, 3H), 1.23–1.35 (m, 10H), 1.62–1.72 (m, 2H), 3.42 (app t, Hz, 2H), 4.58 (s, 2H), 6.56 (app d, Hz, 2H), 6.99–7.16 (m, 4H), 8.17 (app d, Hz, 2H); ¹³C NMR (50 MHz, CDCl₃) 14.1, 19.2, 22.6, 26.9, 29.2, 29.3, 31.7, 51.3, 53.3, 107.4, 116.1 (d Hz), 127.9 (d,J = 8.1 Hz), 130.9 (d, Hz), 144.9, 155.3, 162.3 (d, Hz).

N-(4-Fluorobenzyl)-N-(3-phenylpropyl)pyridin-4-amine 3be. R_f (ethyl acetate: n-hexane: methanol 50 : 33 : 17) 0.48; ¹H NMR (200 MHz, CDCl₃) δ 1.91–2.03 (m, 2H), 2.68 (t, Hz, 2H), 3.43 (app t, J = 7.8 Hz, 2H), 4.54 (s, 2H), 6.44 (app d, Hz, 2H), 6.96–7.36 (m, 9H), 8.15 (bs, 2H); ¹³C NMR (50 MHz, CDCl₃) 28.1, 33.0, 49.9, 52.9, 106.8, 115.8 (d, Hz), 126.3, 127.9 (d, Hz), 128.3, 128.6, 132.1 (d, Hz), 140.7, 147.9, 153.7, 162.1 (d, J = 245.8 Hz).

5.5. Dialkylation of 4-Aminopyridine

To a solution of 4AP (1 mmol) in anhydrous DMSO (2 cm³), kept at rt under N₂, 2 mmol of t-BuOK was added. This mixture was allowed to stir for 20 min at rt; then 2 mmol of alkyl halide was added and the solution was kept under stirring at rt for 4 h. The solution was then mixed with water and extracted with dichloromethane. The solvent was removed under reduced pressure and the mixture was purified by flash chromatography, yielding pure compound 3.

N,N-Di(3phenylpropyl)pyridin-4-amine 3bb. R_f (80% ethyl acetate in dichloromethane) 0.20; ¹H NMR (200 MHz, CD₃CN) δ 1.84–1.99 (m, 4H), 2.65 (t, Hz, 4H), 3.29 (t, Hz, 4H), 6.30 (d, Hz, 2H), 7.17–7.31 (m, 10H), 8.13 (d, Hz, 2H); ¹³C NMR (50 MHz, CD₃CN) δ 28.3, 33.1, 49.5, 106.4, 126.2, 128.3, 128.5, 141.1, 140.7, 152.4.

N,N-Dibenzylpyridin-4-amine 3cc. R_f (80% ethyl acetate in ethanol) 0.32; ¹H NMR (200 MHz, CDCl₃) δ 4.67 (s, 4H), 6.58 (dd,, 1.6 Hz, 2H), 7.19–7.40 (m, 10H), 8.20 (dd,, 1.6 Hz, 2H); ¹³C NMR (50 MHz, CDCl₃) 53.2, 107.1, 126.4, 127.4, 128.9, 136.8, 150.2, 153.9.

N,N–Di(4-fluorobenzyl)pyridin-4-amine 3ee. R_f (80% ethyl acetate in dichloromethane) 0.40; ¹H NMR (200 MHz, CDCl₃) 4.61 (s, 4H), 6.56 (dd,, 1.6 Hz, 2H), 6.99–7.19 (m, 8H), 8.22 (dd, J = 5.0, 1.6 Hz, 2H); ¹³C NMR (50 MHz, CDCl₃) δ 52.5, 107.1, 115.8 (d, Hz), 128.1 (d, Hz), 132.3 (d, Hz), 150.3, 153.6, 162.2 (d, Hz).

N,N-Di(4-trifluoromethylbenzyl)pyridin-4-amine 3ff. R_f (80% ethyl acetate in dichloromethane) 0.25; ¹H NMR (200 MHz, CDCl₃) 4.73 (s, 4H), 6.58 (dd, 1.6 Hz, 2H), 7.36–7.59 (m, 8H), 8.26 (dd, J = 5.0, 1.6 Hz, 2H); ¹³C NMR (50 MHz, CDCl₃) δ 53.1, 107.1, 123.3 (q, Hz), 123.8 (q, Hz), 124.5 (q, Hz), 129.7, 131.4 (q, Hz), 137.7, 150.4, 153.4.

5.6. Biological Assays

5.6.1. Antifungal Assay

Organisms. For the antifungal evaluation, strains obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA), the German Collection of Microorganisms (DSMZ, Braunschweig, Germany), and the Pharmaceutical Microbiology Culture Collection (PMC, Department of Public Health and Infectious Diseases, “Sapienza” University, Rome, Italy) were tested. The strains were Candida albicans (ATCC 10231, ATCC 10261, ATCC 24433, ATCC 90028, 3153, PMC 1002, PMC 1011, and PMC 1030), C. parapsilosis ATCC22019, C. parapsilosis DSM 11224, C. tropicalis DSM 11953, C. tropicalis PMC 0908, C. tropicalis PMC 0910, C. glabrata PMC 0805, C. krusei DSM 6128, and C. krusei PMC 0613, Cryptococcus neoformans (DSM 11959, PMC 2102, PMC 2107, PMC 2111, and PMC 2136), dermatophytes (Trichophyton mentagrophytes DSM 4870, T. mentagrophytes PMC6509, Microsporum gypseum DSM 7303, and M. gypseum PMC 7331). All of the strains were stored and grown in accordance with the procedures of the Clinical and Laboratory Standards Institute (CLSI) [26, 27].

Antifungal Susceptibility Assays. In vitro antifungal susceptibility was evaluated using the CLSI broth microdilution methods [26, 27]. Fluconazole and Amphotericin B were used as reference drugs. The final concentration ranged from 0.125 to 64 μg/mL. The compounds were dissolved previously in DMSO at concentrations 100 times higher than the highest desired test concentration and successively diluted in test medium in accordance with the procedures of the CLSI [28]. Microdilution trays containing 100 μL of serial twofold dilutions of compounds in RPMI 1640 medium (Sigma-Aldrich, St. Louis, MO, USA) were inoculated with an organism suspension adjusted to attain a final inoculum concentration of cells/mL for yeasts and CFU/mL for dermatophytes. The panels were incubated at 35°C and observed for the presence of growth at 48 h (Candida spp.) and 72 h (C. neoformans and dermatophytes).

The minimal inhibitory concentration (MIC) was, for yeasts, the lowest concentration that showed ≥ 50% growth inhibition compared with the growth control and, for dermatophytes, the lowest concentration that showed ≥ 80% growth inhibition compared with the growth control. The MIC₁₀₀ was the lowest drug concentration that prevented 100% of growth with respect to the untreated control. According to CSI protocols, the fluconazole MIC₅₀ and the amphotericin B MIC₁₀₀ were calculated (22,23). The results were expressed as the geometric mean (G M) of the MIC values.

5.6.2. Antiprotozoal Assay

For the evaluation of antiprotozoal and cytotoxic activity an integrated panel of microbial screens and standard screening methodologies were adopted as previously described [29] on the following organisms: chloroquine-resistant P. falciparum K 1-strain; L. infantum MHOM/MA (BE)/67 amastigote stage; suramin-sensitive Trypanosoma brucei Squib-427 strain; Trypanosoma cruzi Tulahuen CL2 (benznidazole-sensitive) strain; human fetal lung fibroblast cells (MRC-5 SV2).

All assays were performed in triplicate. Compounds were tested at 5 concentrations (64, 16, 4, 1, and 0.25 μg/mL) to establish a full dose titration and determine the IC₅₀ (inhibitory concentration 50%). The final in-test concentration of DMSO did not exceed 0.5%, which is known not to interfere with the different assays [29].

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Acknowledgments

This work was financially supported by Miur, Italy. The authors thank Mr. Marco Di Pilato for his support in the experimental part of the work.

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Copyright © 2014 Marta Feroci et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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