Table of Contents
ISRN Stem Cells
Volume 2014, Article ID 625164, 10 pages
Research Article

Embryonic Decellularized Cardiac Scaffold Supports Embryonic Stem Cell Differentiation to Produce Beating Cardiac Tissue

1Division of Cardiovascular Diseases, Department of Medicine, Mayo Clinic, Rochester, MN 55905, USA
2Center for Regenerative Medicine, Mayo Clinic, Rochester, MN 55905, USA
3Division of General Internal Medicine, Mayo Clinic, Rochester, MN 55905, USA
4Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, MN 55905, USA
5Transplant Center, Mayo Clinic, Rochester, MN 55905, USA

Received 12 November 2013; Accepted 4 December 2013; Published 31 March 2014

Academic Editors: R. Dressel and S. M. Hwang

Copyright © 2014 Cree Chamberland et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental A. Characterization of E16.5 embryonic cardiac cells. (A) Quantification of actively dividing cardiomyocytes in E16.5 embryonic hearts. Smooth muscle (SMA) and endothelial (CD31) components of the cell suspension obtained from E16.5 hearts. Percentage of cells expressing CD31 or SMA in the population derived from E16.5 hearts.

Supplemental B. Movie of beating 16.5 dpc cardiac cells recellularized heart. (A) Beating activity in an embryonic heart scaffold incubated with E16.5 cardiac cells for 20 Hr.

Supplemental C. Agitation decellularization produces a more intact cardiac matrix. (A) Heart decellularized using a perfusion protocol with 0.25% SDS and DNase (0.5 mg/ml) solution. (B) Heart decellularized by agitation in a 0.25% SDS and DNase (0.5 mg/ml) solution. Structure is better preserved after the agitation protocol. Main anatomical components of the heart are labeled.

  1. Supplementary Material