Table of Contents
International Scholarly Research Notices
Volume 2014, Article ID 628196, 5 pages
Research Article

Determination of Catechin and Epicatechin Content in Chocolates by High-Performance Liquid Chromatography

Natsol Laboratories Private Limited, 2nd Floor, Research & Development Building, Ramky Commercial Hub, Jawaharlal Nehru Pharma City, Visakhapatnam 531 019, India

Received 11 May 2014; Revised 8 August 2014; Accepted 8 August 2014; Published 29 October 2014

Academic Editor: Isabel C. F. R. Ferreira

Copyright © 2014 Raju V. S. S. Gottumukkala et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A simple and sensitive reversed phase high-performance liquid chromatographic (HPLC) method has been developed for the determination of catechin and epicatechin in cocoa powder and chocolates. The separation was achieved on a reversed phase C 18 column (TARGA) 5 μm by gradient elution with a flow rate of 1.0 mL/minute with an operating temperature of 30°C and detection with a UV-Visible detector was at 280 nm. The method was validated for linearity, precision, intra- and interday precision, and accuracy. The developed method is successfully applied for the determination of catechin and epicatechin content in chocolates. The Godiva brand chocolate contains high concentration of epicatechin.