Construction and Characterization of Recombinant HSV-1 Expressing Early Growth Response-1
Figure 3
Expression profile of Egr-1 induced by infection of SIRC cells. (a) Disparity of Egr-1 induction by different virus strains. Cells were infected by viruses with moi of 5 at 4°C for 1 h for attachment followed by incubation at 37°C with various time points. Total RNA was isolated and subjected to qRT-PCR to measure the Egr-1 expression. (b) Accumulation of Egr-1 transcript from recombinant viruses. Total RNA from 24 h postinfected SIRC cells were purified followed by RT-PCR assays. (c) Overexpression of Egr-1 from recombinant virus. Western blot analyses were performed at the condition described in (b) using anti-Egr-1 Ab.
