Table of Contents
International Scholarly Research Notices
Volume 2014 (2014), Article ID 632672, 6 pages
http://dx.doi.org/10.1155/2014/632672
Research Article

Protective Effects of Quercetin against Dimethoate-Induced Cytotoxicity and Genotoxicity in Allium sativum Test

Gene-Tox Laboratory, Division of Genetics, Department of Zoology, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India

Received 18 March 2014; Accepted 26 May 2014; Published 21 July 2014

Academic Editor: Ana I. Haza

Copyright © 2014 Waseem Ahmad et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. I. Ayed-Boussema, K. Rjiba, N. Mnasri, A. Moussa, and H. Bacha, “Genotoxicity evaluation of dimethoate to experimental mice by micronucleus, chromosome aberration tests, and comet assay,” International Journal of Toxicology, vol. 31, no. 1, pp. 78–85, 2012. View at Publisher · View at Google Scholar · View at Scopus
  2. IPSC Environmental Health Criteria 90, Dimethoate, World Health Organization, Geneva, Switzerland, 1989.
  3. US EPA, “US Environmental Protection Agency: proposed tolerance,” Federal Register, no. 227, pp. 52951–52952, 1983. View at Google Scholar
  4. International Agency for Research on Cancer (IARC), “Miscellaneous pesticides,” IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, vol. 30, pp. 1–424, 1983. View at Google Scholar
  5. T. J. Ellingham, E. A. Christensen, and M. B. Maddock, “In vitro induction of sister chromatid exchanges and chromosomal aberrations in peripheral lymphocytes of the oyster toadfish and American eel,” Environmental Mutagenesis, vol. 8, no. 4, pp. 555–569, 1986. View at Google Scholar · View at Scopus
  6. P. Dolara, M. Salvadori, T. Capobianco, and F. Torricelli, “Sister-chromatid exchanges in human lymphocytes induced by dimethoate, omethoate, deltamethrin, benomyl and their mixture,” Mutation Research—Mutation Research Letters, vol. 283, no. 2, pp. 113–118, 1992. View at Publisher · View at Google Scholar · View at Scopus
  7. A. Bianchi-Santamaria, M. Gobbi, M. Cembran, and A. Arnaboldi, “Human lymphocyte micronucleus genotoxicity test with mixtures of phytochemicals in environmental concentrations,” Mutation Research—Genetic Toxicology and Environmental Mutagenesis, vol. 388, no. 1, pp. 27–32, 1997. View at Publisher · View at Google Scholar · View at Scopus
  8. D. Geetanjali, P. Rita, and P. P. Reddy, “Effect of ascorbic acid in the detoxification of the insecticide dimethoate in the bone marrow erythrocytes of mice,” Food and Chemical Toxicology, vol. 31, no. 6, pp. 435–437, 1993. View at Publisher · View at Google Scholar · View at Scopus
  9. U. Undeger, L. Institóris, O. Siroki, M. Nehéz, and I. Dési, “Simultaneous geno- and immunotoxicological investigations for early detection of organophosphate toxicity in rats,” Ecotoxicology and Environmental Safety, vol. 45, no. 1, pp. 43–48, 2000. View at Publisher · View at Google Scholar · View at Scopus
  10. L. Osaba, A. Aguirre, A. Alonso, and U. Graf, “Genotoxicity testing of six insecticides in two crosses of the Drosophila wing spot test,” Mutation Research—Genetic Toxicology and Environmental Mutagenesis, vol. 439, no. 1, pp. 49–61, 1999. View at Publisher · View at Google Scholar · View at Scopus
  11. Ü. Ündeǧer and N. Başaran, “Effects of pesticides on human peripheral lymphocytes in vitro: induction of DNA damage,” Archives of Toxicology, vol. 79, no. 3, pp. 169–176, 2005. View at Publisher · View at Google Scholar · View at Scopus
  12. L. R. Ferguson, “Antimutagens as cancer chemopreventive agents in the diet,” Mutation Research—Fundamental and Molecular Mechanisms of Mutagenesis, vol. 307, no. 1, pp. 395–410, 1994. View at Publisher · View at Google Scholar · View at Scopus
  13. J. Q. Griffith, C. F. Kreivson, and J. Naghski, Rutin and Related Flavonoids, 1955.
  14. Y. Sakanashi, K. Oyama, H. Matsui et al., “Possible use of quercetin, an antioxidant, for protection of cells suffering from overload of intracellular Ca2+: a model experiment,” Life Sciences, vol. 83, no. 5-6, pp. 164–169, 2008. View at Publisher · View at Google Scholar · View at Scopus
  15. S. C. Bischoff, “Quercetin: potentials in the prevention and therapy of disease,” Current Opinion in Clinical Nutrition and Metabolic Care, vol. 11, no. 6, pp. 733–740, 2008. View at Publisher · View at Google Scholar · View at Scopus
  16. M. Fiorani, R. de Sanctis, P. Menghinello, L. Cucchiarini, B. Cellini, and M. Dacha, “Quercetin prevents glutathione depletion induced by dehydroascorbic acid in rabbit red blood cells,” Free Radical Research, vol. 34, no. 6, pp. 639–648, 2001. View at Google Scholar · View at Scopus
  17. G. W. Plumb, K. R. Price, and G. Williamson, “Antioxidant properties of flavonol glycosides from green beans,” Redox Report, vol. 4, no. 3, pp. 123–127, 1999. View at Publisher · View at Google Scholar · View at Scopus
  18. C. G. M. Heijnen, G. R. M. M. Haenen, F. A. A. van Acker, W. J. F. van der Vijgh, and A. Bast, “Flavonoids as peroxynitrite scavengers: the role of the hydroxyl groups,” Toxicology In Vitro, vol. 15, no. 1, pp. 3–6, 2001. View at Publisher · View at Google Scholar · View at Scopus
  19. M. Kessler, G. Ubeaud, and L. Jung, “Anti- and pro-oxidant activity of rutin and quercetin derivatives,” Journal of Pharmacy and Pharmacology, vol. 55, no. 1, pp. 131–142, 2003. View at Publisher · View at Google Scholar · View at Scopus
  20. R. Fabiani, A. de Bartolomeo, P. Rosignoli, and G. Morozzi, “Antioxidants prevent the lymphocyte DNA damage induced by PMA-stimulated monocytes,” Nutrition and Cancer, vol. 39, no. 2, pp. 284–291, 2001. View at Google Scholar · View at Scopus
  21. D. Anderson, M. M. Dobrzyńska, N. Başaran, A. Başaran, and T.-. Yu, “Flavonoids modulate comet assay responses to food mutagens in human lymphocytes and sperm,” Mutation Research—Fundamental and Molecular Mechanisms of Mutagenesis, vol. 402, no. 1-2, pp. 269–277, 1998. View at Publisher · View at Google Scholar · View at Scopus
  22. S. A. Aherne and N. M. O'Brien, “Lack of effect of the flavonoids, myricetin, quercetin, and rutin, on repair of H2O2-induced DNA single-strand breaks in Caco-2, Hep G2, and V79 cells,” Nutrition and Cancer, vol. 38, no. 1, pp. 106–115, 2000. View at Google Scholar · View at Scopus
  23. K. Horváthová, I. Chalupa, L. Šebová, D. Tóthová, and A. Vachálková, “Protective effect of quercetin and luteolin in human melanoma HMB-2 cells,” Mutation Research—Genetic Toxicology and Environmental Mutagenesis, vol. 565, no. 2, pp. 105–112, 2005. View at Publisher · View at Google Scholar · View at Scopus
  24. Y. Shukla, A. Arora, and P. Taneja, “Antigenotoxic potential of certain dietary constituents,” Teratogenesis Carcinogenesis and Mutagenesis, vol. 23, no. 1, pp. 323–335, 2003. View at Google Scholar · View at Scopus
  25. W. F. Grant and M. F. Salamone, “Comparative mutagenicity of chemicals selected for test in the International program on chemical safety's collaborative study on plant systems for the detection of environmental mutagens,” Mutation Research—Fundamental and Molecular Mechanisms of Mutagenesis, vol. 310, no. 2, pp. 187–209, 1994. View at Publisher · View at Google Scholar · View at Scopus
  26. B. Ateeq, M. A. Farah, M. N. Ali, and W. Ahmad, “Clastogenicity of pentachlorophenol, 2,4-D and butachlor evaluated by Allium root tip test,” Mutation Research—Genetic Toxicology and Environmental Mutagenesis, vol. 514, no. 1-2, pp. 105–113, 2002. View at Publisher · View at Google Scholar · View at Scopus
  27. S. Shaikh, N. Nazam, M. I. Lone, and W. Ahmad, “Dichlorophene and dichlorvos mediated genotoxic and cytotoxic assessment on root meristem cells of Allium cepa,” Science Diliman, no. 1, pp. 13–22, 2012. View at Google Scholar
  28. R. Sudhakar, K. N. Ninge Gowda, and G. Venu, “Mitotic abnormalities induced by silk dyeing industry effluents in the cells of Allium cepa,” Cytologia, vol. 66, no. 3, pp. 235–239, 2001. View at Google Scholar · View at Scopus
  29. L. K. S. Chauhan, P. N. Saxena, and S. K. Gupta, “Effects of deltamethrin on the ultrastructures of the root meristem cells of Allium cepa,” Pesticide Biochemistry and Physiology, vol. 64, no. 3, pp. 135–147, 1999. View at Publisher · View at Google Scholar · View at Scopus
  30. M. H. Schneiderman, W. C. Dewey, and D. P. Highfield, “Inhibition of DNA synthesis in synchronized Chinese hamster cells treated in G1 with cycloheximide,” Experimental Cell Research, vol. 67, no. 1, pp. 147–155, 1971. View at Google Scholar · View at Scopus
  31. B. Novák, J. C. Sible, and J. J. Tyson, “Checkpoints in the cell cycle,” in Encyclopedia of Life Sciences, pp. 1–8, Macmillan Publishers, Nature Publishing Group, 2002, http://www.els.net. View at Google Scholar
  32. V. Smaka-Kincl, P. Stegnar, M. Lovka, and M. J. Toman, “The evaluation of waste, surface and ground water quality using the Allium test procedure,” Mutation Research—Genetic Toxicology, vol. 368, no. 3-4, pp. 171–179, 1996. View at Publisher · View at Google Scholar · View at Scopus
  33. L. K. S. Chauhan and S. K. Gupta, “Combined cytogenetic and ultrastructural effects of substituted urea herbicides and synthetic pyrethroid insecticide on the root meristem cells of Allium cepa,” Pesticide Biochemistry and Physiology, vol. 82, no. 1, pp. 27–35, 2005. View at Publisher · View at Google Scholar · View at Scopus
  34. L. K. S. Chauhan and V. Sundararaman, “Effects of substituted ureas on plant cells. I. Cytological effects of isopruturon on the root meristem cells of A. cepa,” Cytologia, vol. 55, no. 1, pp. 91–98, 1990. View at Publisher · View at Google Scholar
  35. P. Kaur and I. S. Grover, “Cytological effects of some organophosphorus pesticides. II. Meiotic effects,” Cytologia, vol. 50, no. 1, pp. 199–211, 1985. View at Google Scholar · View at Scopus
  36. M. Cabaravdic, “Induction of chromosome aberrations in the Allium cepa test system caused by the exposure of cells to benzo(a) pyrene,” Medicinski Arhiv, vol. 64, no. 4, pp. 215–218, 2010. View at Google Scholar · View at Scopus
  37. B. C. Patil and G. I. Bhat, “A comparative study of MH and EMS in the induction of choromosomal aberrations on lateral root mersitem in Clitoria ternata L.,” Cytologia, vol. 57, no. 2, pp. 259–264, 1992. View at Google Scholar
  38. S. M. Attia, “The impact of quercetin on cisplatin-induced clastogenesis and apoptosis in murine marrow cells,” Mutagenesis, vol. 25, no. 3, pp. 281–288, 2010. View at Publisher · View at Google Scholar · View at Scopus
  39. M. A. Papiez, A. Cierniak, W. Krzysciak et al., “The changes of antioxidant defense system caused by quercetin administration do not lead to DNA damage and apoptosis in the spleen and bone marrow cells of rats,” Food and Chemical Toxicology, vol. 46, no. 9, pp. 3053–3058, 2008. View at Publisher · View at Google Scholar · View at Scopus
  40. M. M. Silva, M. R. Santos, G. Caroço, R. Rocha, G. Justino, and L. Mira, “Structure-antioxidant activity relationships of flavonoids: a re-examination,” Free Radical Research, vol. 36, no. 11, pp. 1219–1227, 2002. View at Google Scholar · View at Scopus
  41. J. E. Brown, H. Khodr, R. C. Hider, and C. A. Rice-Evans, “Structural dependence of flavonoid interactions with Cu2+ ions: implications for their antioxidant properties,” Biochemical Journal, vol. 330, no. 3, pp. 1173–1178, 1998. View at Google Scholar · View at Scopus
  42. J. Terao and M. K. Piskula, “Flavonoids and membrane lipid peroxidation inhibition,” Nutrition, vol. 15, no. 10, pp. 790–791, 1999. View at Publisher · View at Google Scholar · View at Scopus