Modulation of Arachidonic Acid Metabolism in the Rat Kidney by Sulforaphane: Implications for Regulation of Blood Pressure
Figure 4
Inhibition of AA -hydroxylation activity by SF both in vitro and in vivo. In vitro: renal cortical microsomes from untreated Sprague-Dawley rats were incubated with various concentrations of SF in the presence of NADPH. SF-treated microsomes were then used to measure 20-HETE formation. Values, average of 5 replicates, are expressed as % of control (SF-free microsomes). In vivo: male SHR rats (/group) were administered 20 mg/kg or 40 mg/kg SF in their drinking water for 8 weeks and kidneys were harvested at the end of the study. The NADPH-dependent formation of 20-HETE was measured in renal cortical microsomes. Values are expressed as % of control (rats that received SF-free water). All groups were compared using one-way ANOVA followed by multiple comparisons. *Significant difference from control with .
