Modulation of Arachidonic Acid Metabolism in the Rat Kidney by Sulforaphane: Implications for Regulation of Blood Pressure
Figure 5
Effect of SF on kidney expression of CYP4A. (a) Renal CYP4A protein levels in rats (/group) treated for 8 weeks with SF at 20 mg/kg and 40 mg/kg expressed as the percentage of control levels in untreated rats. The immunoreactive proteins were detected via enhanced chemiluminescence and X-ray film imaging and the resultant signals were analyzed by densitometry. The intensity of the band was normalized to a loading control (GADPH signals); then data were expressed as percent of control. (b) The original specimen analyzed by densitometry (control: lanes 1–5, low SF dose: lanes 6–10, and high SF dose: lanes 11–15). *Significant difference from control with .
