Table of Contents
International Scholarly Research Notices
Volume 2014, Article ID 730516, 6 pages
http://dx.doi.org/10.1155/2014/730516
Research Article

Comparative Hepatoprotective Activity of Ethanolic Extracts of Cuscuta australis against Acetaminophen Intoxication in Wistar Rats

1College of Health Sciences, Ladoke Akintola University of Technology, Ogbomoso 4000, Nigeria
2Department of Veterinary Anatomy, University of Jos, Jos 930001, Nigeria
3Department of Morbidity and Forensic Medicine, Obafemi Awolowo University, Ile Ife A234, Nigeria
4Department of Veterinary Public Health, Anatomy & Physiology and Pharmacology, Ibadan 900001, Nigeria

Received 21 March 2014; Revised 2 June 2014; Accepted 24 June 2014; Published 10 September 2014

Academic Editor: Girja S. Shukla

Copyright © 2014 Rachael O. Folarin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study investigates the comparative hepatoprotective activity of crude ethanol extracts of Cuscuta australis against acetaminophen (APAP) intoxication. Thirty-six rats were randomly divided into six groups of 6 replicates: Group 1 which served as control received water. Group 2 was orally administered 835 mg/kg body wt. of paracetamol on day 8. Groups 3 and 4 were orally administered ethanolic extracts of the seed of Cuscuta australis in doses of 125 mg/kg and 250 mg/kg, respectively, for 7 days and then intoxicated as in Group 2 on the 8th day. Groups 5 and 6 received similar oral doses of Cuscuta australis stem extracts for 7 days and then intoxicated as in Groups 3 and 4. Group 2 rats showed severe periportal hepatic necrosis, significantly elevated serum hepatic injury markers, markedly increased lipid peroxidation, and decreased hepatic antioxidant enzymes activities. Remarkably, Cuscuta australis (seed and stem) extract pretreatments in Groups 3, 4, 5, and 6, most especially, the stem extract pretreatment in Groups 5 and 6, improved better the hepatic histoarchitecture, the hepatocellular, and the oxidative stress injury markers in a dose-dependent manner. Conclusively, ethanol extractions of Cuscuta australis stem appear to protect the liver from acetaminophen intoxication better than the seed counterpart.