International Scholarly Research Notices / 2014 / Article / Fig 1

Research Article

Preventive Effects of a Kampo Medicine, Kakkonto, on Inflammatory Responses via the Suppression of Extracellular Signal-Regulated Kinase Phosphorylation in Lipopolysaccharide-Treated Human Gingival Fibroblasts

Figure 1

Effects of kakkonto on HGFs viability. The effect of kakkonto on the viability of HGFs at 24 h. HGFs were plated in 96-well microplates at 10,000 cells/mL, and media containing LPS and kakkonto were added. Cell numbers were evaluated by WST-8 at 24 h. The optical density (OD) was normalized to a relative value of 100% for cells without LPS or kakkonto treatments and is represented as means ± SD ( ). Open circles, treatment without LPS; closed circles, treatment with 10 ng/mL of LPS. and (without kakkonto versus with kakkonto in the absence of LPS). and (without kakkonto versus with kakkonto in the presence of LPS). values were calculated by pairwise comparisons and corrected with the Holm method (16 null hypotheses).

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