Table of Contents
ISRN Oncology
Volume 2014, Article ID 793695, 6 pages
Research Article

The Human Epidermal Growth Factor Receptor 2 Screening Tests for Breast Cancer Suggested by the New Updated Recommendation of the American Society of Clinical Oncology/College of American Pathologists Will Involve a Rise of the In-Situ Hybridization Tests for the European Laboratories of Pathology

Biopathology and Oncology Departments, Institut Jean Godinot, Unicancer, 1 rue du Général Koenig CS80014, 51726 Reims Cedex, France

Received 13 February 2014; Accepted 20 March 2014; Published 22 April 2014

Academic Editors: G. Gatti, G. E. Kim, and T. Yokoe

Copyright © 2014 Christian Garbar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aims. The differences between the 2007 and the 2013 ASCO/CAP HER2 guidelines have been compared. We also discussed the potential consequences in our pathological practice. Material and Methodology. 189 HER2 fluorescence in situ hybridisation (FISH) tests were performed from 1016 preliminary HER2 immunohistochemical tests (IHC). All cases were reviewed and reclassed following the 2007 and 2013 ASCO/CAP recommendations. Results. The 2013 version decreased false-negative IHC (3/118 versus 1/54, ) and created more 2+ IHC (40/186 versus 89/186, ) or more 3+ IHC (9/186 versus 39/186, ). One false-positive IHC was described for the 2013 version (0/9 versus 1/39, ). Equivocal FISH was reduced (8/186 versus 2/186, ). An estimation based on our data for 1000 patients showed a rise of our FISH tests for the control of 2+ IHC (180 tests for the 2007 version versus 274 tests for the 2013 version or FISH work overflow is +52%) and for the control of 2+/3+ IHC (300 for the 2007 version versus 475 for the 2013 version or FISH work overflow is +58%). Conclusions. The new 2013 ASCO/CAP guidelines have detected more HER2 positive cases but have increased the number of FISH tests.