Research Article

The Human Epidermal Growth Factor Receptor 2 Screening Tests for Breast Cancer Suggested by the New Updated Recommendation of the American Society of Clinical Oncology/College of American Pathologists Will Involve a Rise of the In-Situ Hybridization Tests for the European Laboratories of Pathology

Figure 3

A 66-year-old woman with ductal breast carcinoma. IHC classified 2+ with the 2007 ASCO/CAP recommendation and 3+ with the 2013 ASCO/CAP version. Ratio between HER2 (red) and CEN-17 (green) is 1.9. FISH is equivocal for the 2007 system and positive for the 2013 because the mean of HER2 copy is 6.2. HER2 IHC (Clone A0485, Dako, 200x magnification) and HER2 FISH (HER2 IQFISH pharm DX, Dako, 1000x magnification).
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