Comparison of Reverse Transcription Quantitative Real-Time PCR, Flow Cytometry, and Immunohistochemistry for Detection of Monoclonality in Lymphomas

Table 3

RT-qPCR data for 5 B-lymphoid tumor cell lines.

Cellinje	Kappa Cq	Lambda Cq	Kappa noRT Cq	Lambda noRT Cq	mRNA expression	IHC
U698	10.3	13.8	27	23.5	Kappa > Lambda	Kappa
DG75	10.4	15.9	25.2	24.2	Kappa > Lambda	Kappa
MC116	34.8	10.4	Not detected	23.5	Lambda only	Lambda
RPMI8226	12.2	14.4	25.1	22.2	Kappa > Lambda	Lambda
CCRF-SB	12.3	11.2	26.7	24.4	Lambda </≈ Kappa	Kappa
Neg. control	>33	~30

Cq: cycle of quantification (log transcript numbers); noRT: samples without reverse transcriptase; IHC: immunohistochemistry.