Table of Contents
ISRN Spectroscopy
Volume 2014, Article ID 856760, 6 pages
http://dx.doi.org/10.1155/2014/856760
Research Article

Extractive Spectrophotometric Determination of Tenofovir Disoproxil Fumarate Using Acidic Triphenylmethane Dyes

1Department of Chemistry, Jawaharlal Nehru Technology University, Hyderabad 500085, India
2G.Narayanamma Institute of Technology & Science, Hyderabad 500008, India
3Department of Chemistry, Nizam College (O.U.), Hyderabad, Andhra Pradesh 500001, India

Received 13 December 2013; Accepted 5 February 2014; Published 12 March 2014

Academic Editors: R. Fausto and P.-C. Liao

Copyright © 2014 K. Susmitha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Tenofovir disoproxil fumarate is a nucleotide reverse transcriptase inhibitor that has activity against the hepatitis B and HIV viruses. Three simple and sensitive extractive spectrophotometric methods have been described for the assay of tenofovir disoproxil fumarate either in pure form or in pharmaceutical formulations. The developed methods involve formation of colored chloroform extractable ion-pair complexes of the drugs with triphenylmethane dyes, namely, bromothymol blue (BTB), bromophenol blue (BPB), and bromocresol purple (BCP) in acidic medium. The extracted complexes showed absorbance maxima between 410 and 415 nm. Beer’s law is obeyed in the concentration ranges 1.5–25, 1.0–25, and 1.25–25 μg mL−1 with BTB, BPB, and BCP, respectively. The effectc of concentration of dye, pH, and interference of excipients have been studied and optimized. The limits of detection and quantification have been determined. All three methods are validated as per the guidelines of ICH. The methods have been applied to the determination of drug in commercial tablets and results of analysis were validated statistically through recovery studies.