Table of Contents
International Scholarly Research Notices
Volume 2014, Article ID 976264, 7 pages
Research Article

Simultaneous Estimation of Four Antitussive Components from Herbal Cough Syrup by HPTLC

Government College of Pharmacy, Kathora Naka, Amravati 444604, India

Received 10 June 2014; Accepted 16 August 2014; Published 28 October 2014

Academic Editor: Jiiang H. Jeng

Copyright © 2014 Sharada L. Deore et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A new simple, rapid, selective and precise high performance thin layer chromatographic (HPTLC) method has been developed for simultaneous estimation of vasicine, glycyrrhizin, eugenol, and cineole in herbal cough syrup. The retention factors of vasicine, glycyrrhizin, eugenol, and cineole are 0.53, 0.44, 0.75, and 0.77, respectively. Chromatography was performed on 60F254 percolated TLC plate using n-hexane : ethyl acetate : glacial acetic acid (8.5 : 1.0 : 0.5 v/v/v). Methods are validated according to ICH guidelines and can be adopted for the routine analysis of vasicine, glycyrrhizin, eugenol and cineole in herbal cough syrup.