Oral Metformin Treatment Counteracts Adipoinsular Axis Dysfunction in Hypothalamic Obese Rats

Table 1

Rat-specific primers (in alphabetical order) designed and used for real-time PCR analyses.

		GBAN	bp
ACTB	se, 5′-AGCCATGTACGTAGCCATCC-3′	NM_031144	115
	as, 5′-ACCCTCATAGATGGGCACAG-3′
LEP	se, 5′-GAGACCTCCTCCATCTGCTG-3′	NM_013076	192
	as, 5′-CTCAGCATTCAGGGCTAAGG-3′
NPY	se, 5′-TACTCCGCTCTGCGACACTA-3′	NM_012614	115
	as, 5′-GGGCATTTTCTGTGCTTTCT-3′

se: sense; as: antisense; GBAN: GenBank Accession Number; amplicon length, in bp.