Table of Contents
International Scholarly Research Notices
Volume 2015 (2015), Article ID 534065, 7 pages
Research Article

Development of Simultaneous Derivative Spectrophotometric and HPLC Methods for Determination of 17-Beta-Estradiol and Drospirenone in Combined Dosage Form

Department of Analytical Chemistry, Faculty of Pharmacy, Istanbul University, Beyazıt, 34116 Istanbul, Turkey

Received 13 February 2015; Accepted 2 April 2015

Academic Editor: Qi Zhang

Copyright © 2015 Zeynep Aydoğmuş et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Simple, rapid spectrophotometric, and reverse-phase high performance liquid chromatographic methods were developed for the concurrent analysis of 17-beta-estradiol (ESR) and drospirenone (DRS). The spectrophotometric method was based on the determination of first derivative spectra and determined ESR and DRS using the zero-crossing technique at 208 and 282 nm, respectively, in methanol. The linear range was 0.5–32.0 µg·mL−1 for DRS and 0.5–8.0 µg·mL−1 for EST. The limit of detection (LOD) values were 0.14 µg·mL−1 and 0.10 µg·mL−1 and limit of quantification (LOQ) values were 0.42 µg·mL−1 and 0.29 µg·mL−1 for ESR and DRS, respectively. The chromatographic method was based on the separation of both analytes on a C18 column with a mobile phase containing acetonitrile and water (70 : 30, v/v). Detection was performed with a UV-photodiode array detector at 279 nm. The linear range was 0.08–2.5 µg·mL−1 for DRS and 0.23–7.5 µg·mL−1 for EST. LOD values were 0.05 µg·mL−1 and 0.02 µg·mL−1 and LOQ values were 0.15 µg·mL−1 and 0.05 µg·mL−1 for ESR and DRS, respectively. These recommended methods have been applied for the simultaneous determination of ESR and DRS in their tablets.