Development of Simultaneous Derivative Spectrophotometric and HPLC Methods for Determination of 17-Beta-Estradiol and Drospirenone in Combined Dosage Form

Table 1

Results of analytical parameters of proposed methods.

Parameters	Derivative spectrophotometric method		HPLC method
	ESR	DRS	ESR	DRS
Linearity range (µg mL^−1) ()	0.5–8.0	0.5–32.0	0.23–7.5	0.08–2.5
Regression equation^a
Slope	0.014	0.003	45.83	264.95
Intercept	0.009	0.001	1.23	2.208
Correlation coefficient ()	0.9967	0.9998	0.9999	1.0
SD of	0.000	0.000	0.842	2.86
SD of	0.000	0.000	0.71	1.43
LOD (µg mL^−1)	0.14	0.10	0.05	0.02
LOQ (µg mL^−1)	0.42	0.29	0.15	0.05

 ^a (C is the concentration of drug in µg mL⁻¹ for both methods, y is absorbance at λ_max for derivative spectrophotometric method and peak area for HPLC method, is slope, and is intercept) and average of five and six determination points for spectrophotometric and HPLC method, respectively.