Table of Contents
International Scholarly Research Notices
Volume 2017, Article ID 1635780, 5 pages
https://doi.org/10.1155/2017/1635780
Research Article

Use of Genotype MTBDRplus Assay for Diagnosis of Multidrug-Resistant Tuberculosis in Nepal

1Department of Microbiology, Kathmandu College of Science and Technology, Kathmandu, Nepal
2Department of Microbiology, Grande International Hospital, Dhapasi, Kathmandu, Nepal
3Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Nepal
4German-Nepal Tuberculosis Project (GENETUP), Kalimati, Kathmandu, Nepal

Correspondence should be addressed to Narayan Dutt Pant; moc.liamg@7891tnapdn

Received 21 February 2017; Revised 11 May 2017; Accepted 29 May 2017; Published 13 August 2017

Academic Editor: Delia Goletti

Copyright © 2017 Elina Maharjan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The main aims of this study were to study the patterns of mutations in rpoB, katG, and inhA genes in Mycobacterium tuberculosis strains isolated from patients from Nepal and to evaluate the performance of genotype MTBDRplus assay, taking conventional drug susceptibility testing as gold standard for diagnosis of MDR-TB. A total of 69 Mycobacterium tuberculosis strains isolated from 73 smear positive sputum samples from patients suspected of suffering from multidrug-resistant tuberculosis were used in our study. The drug susceptibility pattern of Mycobacterium tuberculosis isolated from these sputum specimens was determined by using genotype MTBDRplus assay taking conventional drug susceptibility testing as reference. The sensitivity and specificity of the genotype MTBDRplus assay for the detection of MDR-TB were found to be 88.7% and 100%, respectively. 88.7% of the rifampicin resistant isolates had mutations in rpoB gene. Similarly, 79.7% and 9.4% of isoniazid resistant isolates had mutations in katG and inhA genes, respectively. Genotype MTBDRplus assay was found to be very rapid and highly sensitive and specific method for diagnosis of MDR-TB and will be very helpful for early diagnosis of MDR-TB in high tuberculosis burden countries.