Research Article

Interleukin-13, but Not Indomethacin, Increases Cysteinyl-Leukotriene Synthesis in Human Lung Macrophages

Figure 2

Enzyme immunoassay data on release of total cysteinyl-leukotrienes (cys-LT, pg/million cells) by macrophages from eight lung tissue donors after culture for 16 hours in the absence or presence of IL-13 (10 ng/mL). Macrophages were then washed and resuspended in fresh medium for a 30 min incubation with no further addition (Cont), with the NSAID indomethacin (Indo, 100 μM), with the calcium ionophore calcimycin (A23187, 1 μM), or with both indomethacin and calcimycin (Indo + A23187). Pretreatment with IL-13 significantly increased spontaneous cys-LT release ( ) and that induced by calcimycin ( ), but neither was affected by indomethacin in either IL-13 treated or untreated cells.
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