Comparison of CD63 Upregulation Induced by NSAIDs on Basophils and Monocytes in Patients with NSAID Hypersensitivity
Figure 1
Detection by flow cytometry of CD63 upregulation on leukocytes activated by NSAIDs. (a) and (b) Targeting of leukocytes: (a) shows at the top CD33 bright cells, the monocytes, CD33 dim cells, polymorphonuclears, and CD33-negative cells, lymphocytes. (b) Shows among anti-IgE-labelled cells, CD33dim cells, the basophils, and C33 bright, the monocytes. The percentage of monocytes among IgE-labelled cells varies from 0 to 50%, depending on the patient. (c) and (d) Activation of basophils: (c) basophils incubated with buffer. (d) Basophils incubated with 1 mg/mL of ASA and upregulation of CD63 on 30% of basophils. (e) and (f) Activation of monocytes and neutrophils. (e) Monocytes at the top, and neutrophils under, incubated with buffer. (f) After incubation with 1 mg/mL of ASA, upregulation of CD63 on 25% of monocytes and 5% of neutrophils.
