Proteolytic Activity Present in House-Dust-Mite Extracts Degrades ENA-78/CXCL5 and Reduces Neutrophil Migration

Figure 1

Effect of HDM extract (HDME) on the release of angiogenic factors. Typical examples of angiogenesis antibody arrays incubated with conditioned medium (CM) of unstimulated BSMC (a) and with CM of BSMC that were stimulated with 10 μg/mL HDME (b). For identification purposes, the corresponding antibody array map is presented in (c). Angiogenic factors are identified by a letter-number code (e.g., IL-8/CXCL8 = f2, ENA78/CXCL5 = h1, VEGF = h3, etc.). Pos = positive control; Neg = negative control; IC1-IC3 = internal controls 1–3, and standard abbreviations are used for the detected angiogenic factors. (d) Time- and dose-dependent reduction of ENA-78/CXCL5 in the presence of CM of BSMC that were stimulated with 2 concentrations of HDM extract (𝑛=10) for either 24 or 72 hours. The same CM did not significantly affect IL-8/CXCL8 (e) and VEGF (f) levels. ^∗∗𝑃<0.005; ^∗∗∗𝑃<0.001; ns = not significant.

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