Cloning and Expression of Ama r 1, as a Novel Allergen of Amaranthus retroflexus Pollen

Figure 2

SDS-PAGE and immunoreactivity of A. retroflexus pollen extract. Lane MW: molecular weight marker (GE Healthcare, Little Chalfont, UK); lane 1: Coomassie Brilliant Blue-stained SDS-PAGE of the crude extract of A. retroflexus pollen (12.5% acrylamide gel); and lane 2: immunoblotting of A. retroflexus pollen extract. The strip was first blotted with A. retroflexus pollen extract and then incubated with pooled sera of patients allergic to A. retroflexus (patients 1, 2, 4, 6, and 8) and detected for IgE reactive protein bands. Natural Ama r 1 is indicated by white arrow.