Fluorine-Tagged 5-Hydroxytryptophan to Investigate Amino Acid Metabolism In Vivo

Table 1

Growth response of tomato seedlings to treatment with low-and high-dose PBS controls as compared to controls that contain only agar-based Murashige and Skoog basal salt mixture growth media. The enzyme AADC catalyzes the reaction of 5-HTP into 5-HT using the cofactor pyridoxal phosphate. Thus, the PBS controls were needed to determine whether the addition of PBS itself to the media had an effect on plant growth. Numbers in the table represent the mean values ± standard deviation. Each value is based on 3 replicates with 4 plants each (12 per treatment). No statistically significant differences between control and the PBS control treatments were found.

Treatment	Stem length	Root length	Leaf biomass	Root biomass
	(cm)	(cm)	(g)	(g)
Controls	5.0 ± 0.6	5.1 ± 1.1	1.0 ± 0.2	3.7 ± 0.9
1 mL PBS	5.0 ± 1.7	6.4 ± 2.0	1.3 ± 0.5	5.5 ± 2.0
5 mL PBS	5.7 ± 1.9	5.8 ± 2.3	1.0 ± 0.5	4.7 ± 2.2

PBS:phosphate-buffered saline.