Review Article

The Aggregation Inhibitor Peptide QBP1 as a Therapeutic Molecule for the Polyglutamine Neurodegenerative Diseases

Figure 3

Phage display screening strategy for the identification of peptides that selectively bind to the expanded polyQ stretch. Phage libraries expressing random 11-amino acid sequences were first screened for their binding to GST-Q62 via 4 rounds of binding, elution, and amplification. Phage clones isolated from the first screening (350 clones) were further screened for their selective binding to pathologic length GST-Q62 compared to normal-length GST-Q19.
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