Role of Charged Residues in the Catalytic Sites of Escherichia coli ATP Synthase

Figure 4

Compensatory effect inserted arginine residue. Membranes were preincubated with Pi at zero, 2.5 or 10 mM concentration as shown, for 60 min at 23°C. Then, NBD-Cl (125 μM) was added and aliquots withdrawn for assay at time intervals as shown. ATPase activity remaining is plotted against time of incubation with NBD-Cl. ○, no Pi added; □, 2.5 mM Pi; Δ, 10 mM Pi. Data taken from [83].

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