Functional Subunits of Eukaryotic Chaperonin CCT/TRiC in Protein Folding

Table 1

Mutations in the conserved ATP binding/hydrolysis domain and phenotypes in GroEL and three subunits of CCT complex.

Subunit	Amino acid replacement	Phenotype	References
GroEL	G35E	Normal	[96]
GroEL	D87N	Lethal	[96]
GroEL	D87K	Lethal	[96]
Cct1p	G45S	CS	[93]
Cct1p	G48E	HS	[92]
Cct1p	D96E	CS	[93]
Cct1p	G423D	HS	[93]
Cct2p	G41E	Lethal	[94]
Cct6p	G38S	Normal	[95]
Cct6p	G41E	TBZ^S, NaCl^S	[95]
Cct6p	G59R	TBZ^SS, NaCl^S	[95]
Cct6p	D89E	TBZ^S	[95]
Cct6p	G90E	TBZ^SS, NaCl^S	[95]
Cct6p	G414E	CS	[95]
Cct6p	G416D	Normal	[95]
Cct6p	G416E	CS	[95]

CS: Cold sensitive; HS: heat sensitive; TBZ^S: Thiabendazole sensitive; TBZ^SS: Thiabendazole-hyper sensitive; NaCl^S: Sodium chloride sensitive.