Role of Flexibility in Protein-DNA-Drug Recognition: The Case of Asp677Gly-Val703Ile Topoisomerase Mutant Hypersensitive to Camptothecin
Figure 2
(a) Time course of the suicide cleavage reaction carried out with the substrate described at the top of the figure. The arrow indicates the preferential cleavage site for the wild-type protein. (b) Percentage of the cleaved DNA substrate plotted as a function of time for the wild-type (black line) and Asp677Gly-Val703Ile (red line) mutant.
